Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 694009
View all Proprotein Convertase 2/PCSK2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
Gly110-Asn638
Accession # P16519

Specificity

Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Proprotein Convertase 1, 7, 9, or rhFurin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Proprotein Convertase 2/PCSK2 Antibody

Detection of Human Proprotein Convertase 2/PCSK2 antibody by Western Blot.

Detection of Human Proprotein Convertase 2/PCSK2 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Human Proprotein Convertase 2/PCSK2 Monoclonal Antibody (Catalog # MAB6018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Proprotein Convertase 2/PCSK2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line.

Proprotein Convertase 2/ PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Proprotein Convertase 2/ PCSK2 Monoclonal Antibody (Catalog # MAB6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Proprotein Convertase 2/PCSK2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 2/PCSK2 (Catalog # 6018-SE), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human brain (cerebellum) tissue

Reviewed Applications

Read 1 review rated 3 using MAB6018 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

References

  1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
  2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
  3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
  4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
  5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Long Name

Proprotein Convertase Subtilisin/Kexin Type 2

Alternate Names

NEC2, PC2, PCSK2, SPC2

Entrez Gene IDs

5126 (Human); 18549 (Mouse); 25121 (Rat)

Gene Symbol

PCSK2

UniProt

P16519 (Human)

Additional Proprotein Convertase 2/PCSK2 Products

Product Documents for Human Proprotein Convertase 2/PCSK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Proprotein Convertase 2/PCSK2 Antibody

For research use only

Citations for Human Proprotein Convertase 2/PCSK2 Antibody

Customer Reviews for Human Proprotein Convertase 2/PCSK2 Antibody (1)

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  • Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human fibroblast and human neural stem cells
    Species: Human
    Verified Customer | Posted 04/08/2019

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Protocols

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