Human Proprotein Convertase 2/PCSK2 Antibody

Catalog # Availability Size / Price Qty
MAB6018
MAB6018-SP
Detection of Human Proprotein Convertase 2/PCSK2 by Western Blot.
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Human Proprotein Convertase 2/PCSK2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Proprotein Convertase 1, 7, 9, or rhFurin is observed.
Source
Monoclonal Mouse IgG2B Clone # 694009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
Gly110-Asn638
Accession # P16519
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 2/PCSK2 (Catalog # 6018-SE), see our available Western blot detection antibodies
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Proprotein Convertase 2/PCSK2 by Western Blot. View Larger

Detection of Human Proprotein Convertase 2/PCSK2 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Human Proprotein Convertase 2/PCSK2 Monoclonal Antibody (Catalog # MAB6018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line. View Larger

Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line. Proprotein Convertase 2/
PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Proprotein Convertase 2/
PCSK2 Monoclonal Antibody (Catalog # MAB6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

References
  1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
  2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
  3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
  4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
  5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.
Long Name
Proprotein Convertase Subtilisin/Kexin Type 2
Entrez Gene IDs
5126 (Human); 18549 (Mouse); 25121 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.94; KEX2-like endoprotease 2; NEC 2; NEC2; NEC2SPC2; neuroendocrine convertase 2; PC2; PC2NEC-2; PCSK2; Prohormone convertase 2; Proprotein Convertase 2; proprotein convertase subtilisin/kexin type 2; SPC2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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