Prostasin, also known as Prss8 and channel activating protease 1, is a 40 kDa serine protease with trypsin-like substrate specificity that is a member of the peptidase S1 family. The enzyme is synthesized from a 343 amino acid (aa) preproenzyme that contains a 29 aa signal peptide, a propeptide (aa 30-32) and a 290 aa mature chain. A second propeptide (aa 323-343) contains a 21 aa transmembrane region (GPI anchor) which can be proteolytically processed to generate a secreted form of the enzyme. The mature chain forms a heterodimer made up of a light chain (aa 33-44) and a heavy chain (aa 45-322) that are connected by disulfide bonds. In addition, aa 45-286 constitute a peptidase S1 domain, and aa 159 is a potential site for N-linked glycosylation. Mature human Prostasin is 79% and 78% aa identical to mature mouse and rat Prostasin, respectively. Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, salivary gland, and pancreas. It activates amiloride-sensitive sodium channels.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 530622
Loading...
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Prostasin/Prss8
Ala33-Gly319
Accession # Q16651
Ala33-Gly319
Accession # Q16651
Specificity
Detects human Prostasin/Prss8 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Prostasin/Prss8 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Prostasin/Prss8 Antibody
Detection of Human Prostasin/Prss8 by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostasin/Prss8 at approximately 40 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Prostasin/Prss8 in Human Prostate.
Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Prostasin/Prss8 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Human prostate tissue under non-reducing conditions only
Sample: Human prostate tissue under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Prostasin/Prss8
Additional Prostasin/Prss8 Products
Product Documents for Human Prostasin/Prss8 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Prostasin/Prss8 Antibody
For research use only
Related Research Areas
Customer Reviews for Human Prostasin/Prss8 Antibody
There are currently no reviews for this product. Be the first to review Human Prostasin/Prss8 Antibody and earn rewards!
Have you used Human Prostasin/Prss8 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...