Human PTGER2 MAb (Clone 607077)

Catalog # Availability Size / Price Qty
PTGER2 in Human Bladder.
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Product Details
Supplemental Products

Human PTGER2 MAb (Clone 607077) Summary

Species Reactivity
Detects human PTGER2 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 607077
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human PTGER2
Accession # P43116
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry PTGER2 in Human Bladder. View Larger

PTGER2 in Human Bladder. PTGER2 was detected in immersion fixed paraffin-embedded sections of human bladder using Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) at 25 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human PTGER2 by Western Blot. View Larger

Detection of Human PTGER2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTGER2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER2

Prostaglandin E2 receptor (PTGER2) is a 53 kDa member of the G-protein coupled receptor family of proteins. Human PTGER2 is 358 amino acids (aa) in length and has the seven putative transmembrane domains characteristic of G-protein coupled receptors. In addition, there are four potential sites for N-linked glycosylation.  Human PTGER2 shares 86% and 83% aa sequence identity with mouse and rat PTGER2, respectively. PTGER2 is present in the vasculature, the gastrointestinal, tracheobronchial, and reproductive tracts, the kidney, and also in the sphincter, dilator, and ciliary muscles in the eye.  PTGER2 is linked to prostaglandin E2-mediated vasodilation and smooth muscle relaxation. It is also involved in the stimulation of secretion in the stomach and small intestine, the control of intraocular pressure, and the regulation of bone resorption.

Long Name
Prostaglandin E2 Receptor 2, Subtype EP2
Entrez Gene IDs
5732 (Human); 19217 (Mouse); 81752 (Rat)
Alternate Names
EP2; PGE receptor EP2 subtype; PGE2 receptor EP2 subtype; prostaglandin E receptor 2 (subtype EP2), 53kD; prostaglandin E receptor 2 (subtype EP2), 53kDa; prostaglandin E2 receptor EP2 subtype; Prostanoid EP2 receptor; PTGER2

Product Datasheets


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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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