Human PTGER3 Antibody
Human PTGER3 Antibody Summary
Accession # P43115
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PTGER3 by Western Blot. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTGER3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
PTGER3 in Human Kidney Tissue. PTGER3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.
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