Human PTGER3 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human PTGER3 by Western Blot.
2 Images
Product Details
Supplemental Products

Human PTGER3 Antibody Summary

Species Reactivity
Detects human PTGER3 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 606609
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human PTGER3
Accession # P43115
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PTGER3 antibody by Western Blot. View Larger

Detection of Human PTGER3 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human PTGER3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTGER3 at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Cannabinoid PTGER3 antibody in HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP antibody by Flow Cytometry. View Larger

Detection of Cannabinoid PTGER3 in HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human PTGER3 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER3

Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.

Long Name
Prostaglandin E2 Receptor 3, Subtype EP3
Entrez Gene IDs
5733 (Human); 19218 (Mouse); 24929 (Rat)
Alternate Names
EP3; EP3EP3e; EP3-I; EP3-II; EP3-III; EP3-IV; MGC141828; MGC141829; MGC27302; PGE receptor EP3 subtype; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; prostaglandin E receotor EP3 subtype 3 isoform; prostaglandin E receptor 3 (subtype EP3); prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); Prostanoid EP3 receptor; PTGER3

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PTGER3 Antibody

There are currently no reviews for this product. Be the first to review Human PTGER3 Antibody and earn rewards!

Have you used Human PTGER3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review