Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 606609
View all PTGER3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human PTGER3
Met1-Arg390
Accession # P43115

Specificity

Detects human PTGER3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PTGER3 Antibody

Detection of Human PTGER3 antibody by Western Blot.

Detection of Human PTGER3 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human PTGER3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTGER3 at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Cannabinoid PTGER3 antibody in HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP antibody by Flow Cytometry.

Detection of Cannabinoid PTGER3 in HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human PTGER3 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PTGER3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP

Western Blot

2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human PTGER3

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTGER3

Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.

Long Name

Prostaglandin E2 Receptor 3, Subtype EP3

Alternate Names

EP3

Entrez Gene IDs

5733 (Human); 19218 (Mouse); 24929 (Rat)

Gene Symbol

PTGER3

UniProt

P43115 (Human)

Additional PTGER3 Products

Product Documents for Human PTGER3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTGER3 Antibody

For research use only

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