Human/Rat MafB Antibody

Catalog # Availability Size / Price Qty
MAB3810
MAB3810-SP
Detection of Human/Rat MafB by Western Blot.
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Product Details
Citations (1)
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Human/Rat MafB Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat MafB at in Western blots. No cross-reactivity with recombinant human (rh) MafF, rhMafG, or rhMafK is observed.
Source
Monoclonal Mouse IgG2B Clone # 372239
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MafB
Met1-Leu323
Accession # Q9Y5Q3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat MafB antibody by Western Blot. View Larger

Detection of Human/Rat MafB by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK were included. A specific band for MafB was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry MafB antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

MafB in HepG2 Human Cell Line. MafB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human MafB antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human MafB by Simple WesternTM. Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for MafB at approximately 53 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafB

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. MafB has been implicated in the regulation of hindbrain development.

Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B
Entrez Gene IDs
9935 (Human); 16658 (Mouse)
Alternate Names
Kreisler (mouse) maf-related leucine zipper homolog; Kreisler maf-related leucine zipper homolog; KRML; KRMLMAFB/Kreisler basic region/leucine zipper transcription factor; MafB; Maf-B; MGC43127; transcription factor MafB; v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

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Citation for Human/Rat MafB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The transcription factor MafB promotes anti-inflammatory M2 polarization and cholesterol efflux in macrophages
    Authors: H Kim
    Sci Rep, 2017;7(1):7591.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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