Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 372239
View all MafB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MafB
Met1-Leu323
Accession # Q9Y5Q3

Specificity

Detects human and rat MafB at in Western blots. No cross-reactivity with recombinant human (rh) MafF, rhMafG, or rhMafK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Rat MafB Antibody

Detection of Human/Rat MafB antibody by Western Blot.

Detection of Human/Rat MafB by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK were included. A specific band for MafB was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
MafB antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

MafB in HepG2 Human Cell Line.

MafB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human MafB antibody by Simple WesternTM.

Detection of Human MafB by Simple WesternTM.

Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for MafB at approximately 53 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Rat MafB Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Simple Western

5 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MafB

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. MafB has been implicated in the regulation of hindbrain development.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B

Alternate Names

KRML

Entrez Gene IDs

9935 (Human); 16658 (Mouse)

Gene Symbol

MAFB

UniProt

Q9Y5Q3

Additional MafB Products

Product Documents for Human/Rat MafB Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat MafB Antibody

For research use only

Citations for Human/Rat MafB Antibody

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Protocols

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