Human/Rat Myoglobin Antibody

Catalog # Availability Size / Price Qty
MAB9720-100
MAB9720-SP
Detection of Human and Rat Myoglobin by Western Blot.
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Human/Rat Myoglobin Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human Myoglobin in direct ELISAs. Detects human and rat Myoglobin in Western blots. In Western blots, no detection of mouse myglobin was observed.
Source
Monoclonal Mouse IgG2A Clone # 974102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified human cardiac Myoglobin antigen from human heart
Accession # P02144
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Rat Myoglobin antibody by Western Blot. View Larger

Detection of Human and Rat Myoglobin by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue (negative control), rat heart tissue, and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Myoglobin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human and Rat Myoglobin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Rat Myoglobin by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, rat heart tissue, and mouse heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Myoglobin at approximately 22 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myoglobin

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.

Entrez Gene IDs
4151 (Human); 17189 (Mouse); 59108 (Rat)
Alternate Names
MB; MGC13548; Myoglobin; PVALB

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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