Human/Rat Neurogenin-2 Antibody

Catalog # Availability Size / Price Qty
MAB3314
MAB3314-SP
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Human/Rat Neurogenin-2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detect human and rat Neurogenin‑2.
Source
Monoclonal Mouse IgG2A Clone # 7G4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Neurogenin-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurogenin-2

Neurogenin-2 (NgN2) is a member of neuroD-related bHLH transcription factor family. It is required for neurogenesis and can be used as a marker for cells of neuronal lineage.

Entrez Gene IDs
63973 (Human); 11924 (Mouse); 295475 (Rat)
Alternate Names
Atoh4; BHLHA8; bHLHa8MGC46562; Class A basic helix-loop-helix protein 8; Math4A; NEUROG2; neurogenin 2; Neurogenin2; Neurogenin-2; NGN2; ngn-2; Protein atonal homolog 4

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Citations for Human/Rat Neurogenin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset
    Authors: X Shi, Y Zhuang, Z Chen, M Xu, J Kuang, XL Sun, L Gao, X Kuang, H Zhang, W Li, SZH Wong, C Liu, L Liu, D Jiang, D Pei, Y Lin, QF Wu
    Science Advances, 2022;8(46):eabq2987.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cascade diversification directs generation of neuronal diversity in the hypothalamus
    Authors: YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu
    Cell Stem Cell, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors
    Authors: S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells
    Authors: VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski
    J. Neurosci., 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Conversion of adult human fibroblasts into neural precursor cells using chemically modified mRNA
    Authors: B Connor, E Firmin, A McCaughey-, R Monk, K Lee, S Liot, J Geiger, C Rudolph, K Jones
    Heliyon, 2018;4(11):e00918.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Notch signaling differentially regulates Atoh7 and Neurog2 in the distal mouse retina.
    Authors: Maurer K, Riesenberg A, Brown N
    Development, 2014;141(16):3243-54.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007;505(1):73-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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