Human/Rat PAK7 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Rat PAK7 by Western Blot.
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Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human/Rat PAK7 Antibody Summary

Species Reactivity
Human, Rat
Detects human and rat PAK7 in Western blots.
Monoclonal Mouse IgG2B Clone # 444703
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PAK7
Accession # Q9P286
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat PAK7 antibody by Western Blot. View Larger

Detection of Human/Rat PAK7 by Western Blot. Western blot shows lysates of human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK7 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry PAK7 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

PAK7 in Human Brain. PAK7 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to Purkinje cell bodies and dendrites. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK7

PAK7 (p21-activated kinase 7), also called PAK5, is a neuronal member of the PAK-II subfamily within the STE Ser/Thr protein kinase family. In the portion used as immunogen, human PAK7 shares 95% aa sequence identity with rat PAK7. This portion contains a CDC42/Rac1 interactive binding (CRIB) motif and potential phosphorylation sites at Ser104 and Thr107. In addition, PAK7 also interacts with RhoD and RhoH, directing its subcellular localization. PAK7 can be located in the mitochondrion, where it protects cells from apoptosis, or in the nucleus.

Long Name
p21/Cdc42/Rac1-activated Kinase 7
Entrez Gene IDs
57144 (Human); 241656 (Mouse); 311450 (Rat)
Alternate Names

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Citations for Human/Rat PAK7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PAK5-mediated AIF phosphorylation inhibits its nuclear translocation and promotes breast cancer tumorigenesis
    Authors: Y Xing, Y Li, B Hu, F Han, X Zhao, H Zhang, Y Li, D Li, J Li, F Jin, F Li
    International journal of biological sciences, 2021;17(5):1315-1327.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. P21-Activated Kinase Inhibitors FRAX486 and IPA3: Inhibition of Prostate Stromal Cell Growth and Effects on Smooth Muscle Contraction in the Human Prostate
    Authors: Y Wang, C Gratzke, A Tamalunas, N Wiemer, A Ciotkowska, B Rutz, R Waidelich, F Strittmatt, C Liu, CG Stief, M Hennenberg
    PLoS ONE, 2016;11(4):e0153312.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Human/Rat PAK7 Antibody
By Anonymous on 12/18/2021
Application: IHC Sample Tested: Lymph node tissue Species: Human