Detection of Human Rit2 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and human brain (hypothalamus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Rit2 Monoclonal Antibody (Catalog # MAB6094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25 kDa member of the small GTPase superfamily and Ras family. Human Rit2 is 217 amino acids (aa) in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11 aa substitution for residues 143‑153 found in isoform 1 and a deletion of aa 154‑217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C‑terminal binding motif.
Ras-like without CAAX 2
Entrez Gene IDs:
6014 (Human); 19762 (Mouse); 291713 (Rat)
GTP-binding protein Rit2; GTP-binding protein Roc2; Ras-like protein expressed in neurons; Ras-like without CAAX 2; Ras-like without CAAX protein 2; RIBA; Ric (Drosophila)-like, expressed in neurons; Ric-like, expressed in neurons; RIN; Rit2; ROC2
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