Human Rit2 Antibody Summary
Accession # Q99578
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Rit2 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (hypothalamus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Rit2 Monoclonal Antibody (Catalog # MAB6094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25 kDa member of the small GTPase superfamily and Ras family. Human Rit2 is 217 amino acids (aa) in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11 aa substitution for residues 143‑153 found in isoform 1 and a deletion of aa 154‑217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C‑terminal binding motif.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Rit2 Antibody
There are currently no reviews for this product. Be the first to review Human Rit2 Antibody and earn rewards!
Have you used Human Rit2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image