Detection of Human RUNX1/CBFA2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RUNX1/CBFA2 in K562 Human Cell Line.
RUNX1/CBFA2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
RUNX1 (runt-related transcription factor 1), also known as CBFA2 (core-binding factor alpha 2) or AML1 (acute myelogenous leukemia 1), is
a heterodimeric transcription factor that plays a role in normal
hematopoiesis. Multiple splice variants exist. Defects in RUNX1 are associated with several types of
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