Human S100P Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human S100P in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) S100A10 or rhS100B is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 357517
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human S100P
    Met1-Lys95
    Accession # P25815
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Knockout Validated
    S100P is specifically detected in HT‑29 human colon adenocarcinoma parental cell line but is not detectable in S100P knockout HT‑29 cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human S100P by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma parental cell line and S100P knockout HT‑29 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT‑29cell line, but is not detectable in knockout HT‑29 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
S100P in NTera-2 Human Cell Line. S100P was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100P

Human S100P is a 22 kDa, homodimeric member of the S100 family of calcium-binding proteins. The S100 family currently has more than 20 members and belongs to the EF-hand superfamily of molecules. S100P is 95 aa in length and contains short, sequential modules. There is an N-terminal alpha -helix, a unique EF-hand motif, an alpha -helix, a linker region, an alpha -helix, a classic EF-hand motif and a C-terminal alpha -helix. The EF-hand motif binds calcium, which likely alters molecular conformation. The rearranged S100P now binds ligand with the linker region. Human S100P shares 47% aa sequence identity with rat S100P.

  • Long Name:
    S100 Calcium Binding Protein P
  • Entrez Gene IDs:
    6286 (Human)
  • Alternate Names:
    MIG9; migration-inducing gene 9; Protein S100-E; S100 calcium binding protein P; S100 calcium-binding protein Pprotein S100-P; S100E; S100P
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Novel quantitative analysis of the S100P protein combined with endoscopic ultrasound-guided fine needle aspiration cytology in the diagnosis of pancreatic adenocarcinoma
    Authors: M Chiba, H Imazu, M Kato, K Ikeda, H Arakawa, T Kato, K Sumiyama, S Homma
    Oncol. Rep, 2017;0(0):.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA detection
  2. Dichotomy in intrahepatic cholangiocarcinomas based on histologic similarities to hilar cholangiocarcinomas
    Authors: M Akita, K Fujikura, T Ajiki, T Fukumoto, K Otani, T Azuma, T Itoh, Y Ku, Y Zen
    Mod. Pathol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. S100P expression in response to sex steroids during the implantation window in human endometrium.
    Authors: Zhang, Dan, Ma, Chengbin, Sun, Xiaoli, Xia, Hexia, Zhang, Wei
    Reprod Biol Endocrinol, 2012;10(0):106.
    Species: Human
    Sample Type: Whole Cells
    Application: in-cell western
  4. S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
    Clin. Cancer Res., 2012;18(16):4356-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Morphoproteomic profile of mTOR, Ras/Raf kinase/ERK, and NF-kappaB pathways in human gastric adenocarcinoma.
    Authors: Feng W, Brown RE, Trung CD
    Ann. Clin. Lab. Sci., 2008;38(3):195-209.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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