Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin A1/alpha 1-Antitrypsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha 1‑Antitrypsin
Glu25-Lys418
Accession # P01009

Specificity

Detects human Serpin A1/ alpha 1‑Antitrypsin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Serpin A1/ alpha 1‑Antitrypsin Antibody

Detection of Human Serpin A1/a1-Antitrypsin antibody by Western Blot.

Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot.

Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serpin A1/a1-Antitrypsin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Serpin A1/ alpha 1‑Antitrypsin in HepG2 Human Cell Line.

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Serpin A1/a1-Antitrypsin antibody by Simple WesternTM.

Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM.

Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Serpin A1/a1-Antitrypsin antibody by Simple WesternTM.

Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serpin A1/a1-Antitrypsin Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

Serpin A1/ alpha 1‑Antitrypsin Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in Serpin A1/a1-Antitrypsin knockout (KO) HepG2 cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 9 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Shows Human Serpin A1/a1-Antitrypsin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Serpin A1/ alpha 1‑Antitrypsin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Serpin A1/a1-Antitrypsin HepG2 knockout cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-55 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin A1/ alpha 1‑Antitrypsin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A1/ alpha 1-Antitrypsin (Catalog # 1268-PI), see our available Western blot detection antibodies

Knockout Validated

Serpin A1 is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Serpin A1 knockout HepG2 cell line.

Simple Western

10 µg/mL
Sample: Human plasma and human kidney tissue

Western Blot

0.1 µg/mL
Sample: Human plasma, human lung tissue, and human kidney tissue

Reviewed Applications

Read 3 reviews rated 4.3 using AF1268 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A1/alpha 1-Antitrypsin

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency (3).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Barbour, K.W. et al. (2002) Genomics 80:515.
  3. Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

UniProt

P01009

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Human Serpin A1/ alpha 1‑Antitrypsin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin A1/ alpha 1‑Antitrypsin Antibody

For research use only

Citations for Human Serpin A1/ alpha 1‑Antitrypsin Antibody

Customer Reviews for Human Serpin A1/ alpha 1‑Antitrypsin Antibody (3)

4.3 out of 5
3 Customer Ratings
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  • Human Serpin A1/ alpha 1-Antitrypsin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver cells
    Species: Human
    Verified Customer | Posted 08/26/2025
  • Human Serpin A1/ alpha 1-Antitrypsin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Human Serpin A1/ alpha 1‑Antitrypsin Antibody
    Name: Daniel Kanmert
    Application: ELISA
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 04/26/2016

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