Human SMPD1 Antibody
Human SMPD1 Antibody Summary
Accession # NP_000534
(Catalog # 5348-PD), see our available Western blot detection antibodies.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.
- Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
- Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
- Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
- Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.
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30 micrograms of proteins per lane. Nitrocellulose membrane. 5% milk/PBS-Tween was used for blocking. Antibody dilution: 1:1000 in 1% milk/PBS-Tween. Lane 1=wild-type cells, lane 2 and 3 show two different knock-out clones obtained by CRISPR/Cas9 genome editing.
A loading control (GAPDH) is also shown.