Human SNAP25 Antibody

Name: Human SNAP25 Antibody
Brand: R&D Systems
SKU: MAB11729

R&D Systems | Catalog # MAB11729

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Product Documents

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1079626

View all SNAP25 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P60880

Specificity

Detects a synthetic peptide specific for Human SNAP-25 around amino acid 30 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human SNAP25 Antibody

Detection of Human SNAP25 by Western Blot.

Western Blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SNAP25 Monoclonal Antibody (Catalog # MAB11729) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SNAP25 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of SNAP25 in Human Hypothalamus.

SNAP25 was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Mouse Anti-Human SNAP25 Monoclonal Antibody (Catalog # MAB11729) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synapses. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of SNAP25 in Human Brain Cortex.

SNAP25 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human SNAP25 Monoclonal Antibody (Catalog # MAB11729) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the vesicles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SNAP25 Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus and human brain cortex

Western Blot

2 µg/mL
Sample: Human cerebellum tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SNAP25

SNAP25 (Synaptosomal associated protein of 25 kDa; also SUP) is a cytosolic 24-29 kDa member of the SNAP25 family of proteins. It is expressed in neurons, beta ‑cells, and adrenal chromaffin cells. SNAP25 is involved in vesicle exocytosis, where two presynaptic SNAP25, and one syntaxin‑1 molecule associates with vesicle membrane VAMP2 to form a alpha ‑helix via a zipper‑like mechanism. This approximates two membranes, which subsequently fuse to create a pore. Human SNAP25b is 206 amino acids (aa) in length. It contains two t‑SNARE coiled‑coil homology domains (aa 19‑81 and 140‑202) and a proteolytic cleavage site at Arg180:Ile181. Palmitoylation occurs between aa 85‑92; phosphorylation occurs on Thr138. There are three isoform variants. One shows nine aa changes over aa 58‑89 (isoform a), a second shows a 30 aa substitution for aa 1‑94, while a third contains an alternative start site at Met64. Full‑length human and mouse SNAP25 are identical in amino acid sequence.

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Entrez Gene IDs

6616 (Human); 20614 (Mouse); 25012 (Rat)

Gene Symbol

SNAP25

UniProt

P60880 (Human)

Additional SNAP25 Products

Product Documents for Human SNAP25 Antibody

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Certificate of Analysis

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Product Specific Notices for Human SNAP25 Antibody

For research use only

Related Research Areas

Neuronal Markers

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