Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Avian - Chicken, Chicken, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, FACS, IHC-F, In Situ, In Situ Hybridization, Proximity Ligation Assay (PLA)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693

Specificity

Detects human SOX10 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX10 Antibody

SOX10 in SK‑Mel‑28 Human Cell Line.

SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SOX10 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX10 in BG01V Human Embryonic Stem Cells.

SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX10 in Human Melanoma Tissue.

SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse SOX10 by Immunohistochemistry

Detection of Mouse SOX10 by Immunohistochemistry

PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

Adult dynamin 2-depleted Schwann cells are replaced by dynamin 2-positive Schwann cells in remyelinated nerves.(A) Immunostainings for DNM2, SOX10 (SCs) and YFP (reporter-recombined SCs) on SN cross-sections of control* (MpzCreERT2:Rosa26-stoploxP/loxP-YFP) and P0ERT2-Dnm2KO* (MpzCreERT2:Dnm2loxP/loxP: Rosa26-stoploxP/loxP-YFP) mice at 4 wpT and 14 wpT. White arrows: YFP-recombined SCs that express DNM2 (in controls at 4 wpt and 14 wpt); black arrowheads: YFP-recombined SCs lacking DNM2 (in mutants at 4 wpt); white arrowheads: Non-recombined SCs expressing DNM2 (in controls and mutants at 4 wpt and 14 wpt). Scale bar = 25 μm for entire panel. (B) Quantification related to (A). Percentage of YFP+ cells among SOX10+ SCs/SN cross-sections at 4 wpT, 6 wpT, and 14 wpT. N = 3 mice/genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (C) Western blot analysis of cleaved caspase 3 (cC3) in SN lysates of control* and P0ERT2-Dnm2KO* mice at 4 wpT, 6 wpT and 14 wpT. Full-length blots in Supplementary file 1F. (D) Quantification referring to (C). Control average was set to 1. N = 3 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. Results in graphs represent means ±s.e.m.; ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence

Schwann cells lacking dynamin 2 show impaired cell cycle progression, reduced mitosis rate, and cytokinesis defects.(A) Schematic representation of the cell cycle phases marked by Ki-67 and EdU. (B) EdU-labeling, combined with immunostainings for Ki-67 and SOX10 on control and P0-Dnm2KO SN cross-sections at P5. Arrows: EdU+ Ki-67+ SOX10+ SCs, arrowheads: Ki-67+ SOX10+ SCs. Scale bar = 25 μm for entire panel. (C) Quantification of (B). Percentage of EdU+ SCs/SN cross-sections. N = 5 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (D) Quantification of (B). Percentage of Ki-67+ SCs/SN cross-sections at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (E) Quantification of (B). Percentage of EdU+ among Ki-67+ SCs/SN cross-section at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (F) Quantification of mitotic events in cultured mouse SCs isolated from MpzCre:Rosa26-stoploxP/loxP-YFP (control*) and MpzCre:Dnm2loxP/loxP:Rosa26-stoploxP/loxP-YFP (P0-Dnm2KO*) SNs at P1, monitored by time-lapse microscopy for 24 hr. Each data point represents one individual animal (at least 42 cells/animal analyzed). N = 5 controls and seven mutant mice, two-tailed unpaired Student´s t-test. (G) Quantification of mitosis duration (minutes) of SCs of control* and P0-Dnm2KO* mice, monitored by time-lapse microscopy for 24 hr. Each data point represents one cell derived from a total of 5 control and seven mutant mice. Cells derived from each animal were isolated and analyzed separately, but pooled in one graph; two-tailed unpaired Student´s t-test. (H) Exemplary picture of a multinucleated Dnm2KO SCs after 48 hr in culture. Scale bar = 25 μm. (I) Quantification of multinucleated cells in control and Dnm2KO SCs after 24 hr, 48 hr and 76 hr in culture. N = 6 mice/genotype for 48 hr; N = 6 control* and n = 5 P0-Dnm2KO* mice for 24 hr and 72 hr, two-Way ANOVA with Sidak’s multiple comparisons test. (J) Exemplary time-lapse images of the mitosis of control and Dnm2KO SCs (YFP+ cells). Arrowheads: cell body of dividing SCs, black arrows: cytokinesis site. (K) Representative time-lapse images of Dnm2KO SC (YFP+) failing cytokinesis. The SC is undergoing mitosis, but fails to divide (arrowheads point to the cell body). Results in graphs represent means ±s.e.m.; *p<0.05, ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunohistochemistry

Detection of Mouse SOX10 by Immunohistochemistry

PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunohistochemistry

Detection of Mouse SOX10 by Immunohistochemistry

Filamentous actin is increased in Nedd4 knockdown&Nedd4−/− NCCs. e Representative images of migrating trunk NCCs within somites (S) 8–10 in control&Wnt1-Cre; Nedd4fl/fl E9.5 embryos. Whole embryos with 20–23 somites stained with anti-SOX10 (green)&anti-CD31 antibodies (red) to recognise NCCs&blood vessels. Dashed line marks the ventral limit of the dorsal aorta (DA) which was used to normalise migration distance of pioneering NCCs (arrowhead). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440627), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOX10 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse SOX10 by Immunocytochemistry/ Immunofluorescence

Mice lacking dynamin 2 in Schwann cells display characteristics of inflammation and Schwann cell death. (E) (Left) Immunostainings of controls and P0-Dnm2KO SN cross-sections for cleaved-caspase 3 (cC3) in combination with the SC marker SOX10 at P5. (Right) Percentage of cC3+ SOX10+ SCs at P5 and P14. N = 5 mice/time point and genotype, two-tailed unpaired Student’s t-test. Scale bar = 25 μm for entire panel. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SOX10 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cell line and immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human SOX10

Reviewed Applications

Read 9 reviews rated 4.4 using AF2864 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX10

SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Entrez Gene IDs

6663 (Human)

Gene Symbol

SOX10

UniProt

P56693

Additional SOX10 Products

Product Documents for Human SOX10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX10 Antibody

For research use only

Citations for Human SOX10 Antibody

Customer Reviews for Human SOX10 Antibody (9)

4.4 out of 5
9 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • Human SOX10 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: human iPS cells
    Verified Customer | Posted 02/06/2020
    Generated cells of oligodendrocytes lineage from iPS cells
    Human SOX10 Antibody AF2864
  • Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 10/29/2019
    Human SOX10 Antibody AF2864
  • Human SOX10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Embryonic spinal cord
    Species: Mouse
    Verified Customer | Posted 05/09/2019
    Human SOX10 Antibody AF2864
  • Human SOX10 Antibody
    Name: Kelli Jordan
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 02/26/2019
    Glial cells in the pancreas. Pink = Sox10; Green = GFAP; blue = DAPI
    Antigen retrieval: HIER, pH 6 Block: 1 hour in 2% normal donkey serum, 2 mg/ml BSA; 0.2% triton-x Primary: 10 ug/ml in blocking buffer, overnight at 4C Secondary: Donkey anti-goat DyLight 650, 1:300 for 1 hour
    Human SOX10 Antibody AF2864
  • Human SOX10 Antibody
    Name: Jennifer Taggart
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 08/20/2018
  • Human SOX10 Antibody
    Name: shelley Cutrone
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 08/20/2018
  • Human SOX10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 06/20/2018
    This antibody worked beautifully at 1:1000 in adult (P25) mouse brain perfused with 4% paraformaldehyde, 30um free floating sections. 1. Antigen Retrieval: 100mM Sodium Citrate pH6.0, 0.05% 2. Rinse sections PBS once 3. Block in 5% NDS in PBSTx 1% RT for 1 hour 4. Primary Antibody (1:1000) in 1% NDS in PBSTx 0.3% (Incubate RT overnight on shaker) 5. Wash 3x 10 min PBS 6. Secondary Antibody 1:600 in 1% NDS in PBSTx 0.3% (Incubate RT 2h on shaker) 7. Wash 3x 10 min PBS 8. Mount on slides in water and let dry 9. Coverslip using Prolong Gold
    Human SOX10 Antibody AF2864
  • Human SOX10 Antibody
    Name: Venkatkrishnan Sundaram
    Application: Immunohistochemistry
    Sample Tested: Sciatic nerve
    Species: Mouse
    Verified Customer | Posted 11/02/2017
    Cryosection of mouse sciatic nerve 10µm thick. Tissue fixed with 4% PFA after dissection, inclusion in OCT. Storage at -80°C. Thawed sections at RT are treated with TritonX100 0.1% Tween 20 0.1% in PBS 1X for half an hour before blocking and primary antibody.
    Human SOX10 Antibody AF2864
  • Human SOX10 Antibody
    Name: luciana frick
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: sciatic nerve culture
    Species: Mouse
    Verified Customer | Posted 07/22/2017
    Cell culture from mouse sciatic nerves, Sox10+ cells are Schwann cells. Standard immunostaining, 5ug/ml of goat anti sox10 antibody. Dapi counterstaining.
    Human SOX10 Antibody AF2864

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