Human SOX10 Antibody

(9 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX10 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX10
    Met1-Ala118
    Accession # P56693
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human SOX10
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX10
SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.
  • Long Name:
    Transcription Factor SOX10
  • Entrez Gene IDs:
    6663 (Human)
  • Alternate Names:
    DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells
    Authors: Yannick Poitelon
    Nat Neurosci, 2016;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: IP
  2. Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay
    Authors: A Blokzijl, LE Chen, SM Gustafsdot, J Vuu, G Ullenhag, O Kämpe, U Landegren, M Kamali-Mog, H Hedstrand
    PLoS ONE, 2016;11(4):e0154214.
    Species: Human
    Sample Type: Serum
    Application: Proximity Ligation Assay
  3. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model.
    Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R
    Sci Rep, 2015;5(0):8468.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  4. Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo.
    Authors: Najm F, Madhavan M, Zaremba A, Shick E, Karl R, Factor D, Miller T, Nevin Z, Kantor C, Sargent A, Quick K, Schlatzer D, Tang H, Papoian R, Brimacombe K, Shen M, Boxer M, Jadhav A, Robinson A, Podojil J, Miller S, Miller R, Tesar P
    Nature, 2015;522(7555):216-20.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  5. Dynamic regulation of Schwann cell enhancers after peripheral nerve injury.
    Authors: Hung H, Sun G, Keles S, Svaren J
    J Biol Chem, 2015;290(11):6937-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  6. Migrating cells mediate long-range WNT signaling.
    Authors: Serralbo, Olivier, Marcelle, Christop
    Development, 2014;141(10):2057-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad.
    Authors: Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H
    Cell Stem Cell, 2008;2(0):392-403.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
    Authors: Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P
    Am J Hum Genet, 0;100(4):617-634.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human SOX10 Antibody AF2864
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Very Good
 IHC Mouse Anonymous 11/02/2017
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Image Details
Immunohistochemistry Human SOX10 Antibody AF2864
Immunohistochemistry: Human SOX10 Antibody [AF2864]

Summary

ApplicationImmunohistochemistry
Sample TestedSciatic nerve
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCryosection of mouse sciatic nerve 10µm thick. Tissue fixed with 4% PFA after dissection, inclusion in OCT. Storage at -80°C. Thawed sections at RT are treated with TritonX100 0.1% Tween 20 0.1% in PBS 1X for half an hour before blocking and primary antibody.
Immunocytochemistry/Immunofluorescence Human SOX10 Antibody AF2864
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Very Good
 ICC/IF Mouse Anonymous 07/22/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human SOX10 Antibody AF2864
Immunocytochemistry/Immunofluorescence: Human SOX10 Antibody [AF2864]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedsciatic nerve culture
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCell culture from mouse sciatic nerves, Sox10+ cells are Schwann cells. Standard immunostaining, 5ug/ml of goat anti sox10 antibody. Dapi counterstaining.

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