Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody

Catalog # Availability Size / Price Qty
AF5924
AF5924-SP
Detection of Human ST6GAL1 by Western Blot.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)

Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Gal Sialyltransferase 1/ST6GAL1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Gal Sialyltransferase 1/ST6GAL1
Lys27-Cys406
Accession # P15907
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ST6GAL1 by Western Blot. View Larger

Detection of Human ST6GAL1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ST6GAL1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human ST6 Gal Sialyltransferase 1/ST6GAL1 by Simple WesternTM. View Larger

Detection of Human ST6 Gal Sialyltransferase 1/ST6GAL1 by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

References
  1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
  2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
  4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
  5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
  6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
  7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
  8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  9. Kitazume, S. et al. (2009) Glycobiology 19:479.
Long Name
ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1
Entrez Gene IDs
6480 (Human); 20440 (Mouse); 25197 (Rat)
Alternate Names
Alpha 2,6-ST; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CD75; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; EC 2.4.99.1; MGC48859; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1; SIAT1; SIAT1Alpha 2,6-ST 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N

Product Datasheets

Citations for Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Transfer of Functional Cargo in Exomeres
    Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
    Cell Rep, 2019;0(0):.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot
  2. Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death
    Authors: CM Britain, AT Holdbrooks, JC Anderson, CD Willey, SL Bellis
    J Ovarian Res, 2018;11(1):12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling
    Authors: RB Jones, KA Dorsett, AB Hjelmeland, SL Bellis
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  4. Unravelling the Role of O-glycans in Influenza A Virus Infection
    Authors: J Mayr, K Lau, JCC Lai, IA Gagarinov, Y Shi, S McAtamney, RWY Chan, J Nicholls, M von Itzste, T Haselhorst
    Sci Rep, 2018;8(1):16382.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
    Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  6. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
    Authors: AT Holdbrooks, CM Britain, SL Bellis
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. B-cell-independent sialylation of IgG
    Authors: Mark B Jones
    Proc Natl Acad Sci USA, 2016;113(26):7207-12.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans.
    Authors: Suzuki O, Abe M
    Int J Oncol, 2014;44(5):1433-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. ST6Gal-I sialyltransferase confers cisplatin resistance in ovarian tumor cells.
    Authors: Schultz, Matthew, Swindall, Amanda F, Wright, John W, Sztul, Elizabet, Landen, Charles, Bellis, Susan L
    J Ovarian Res, 2013;6(1):25.
  11. ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines.
    Authors: Swindall A, Londono-Joshi A, Schultz M, Fineberg N, Buchsbaum D, Bellis S
    Cancer Res, 2013;73(7):2368-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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Reviews for Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody

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Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody
By Anonymous on 03/21/2018
Application: ICC/IF Sample Tested: ST6GAL1 transfected CHO cells Species: Mouse

Used this Ab for both Western and immunofluorescence on mouse ST6GAL1 transfected CHO cells. Fixed cells with 4% Paraformaldehyde and permeabilized with 0.5% saponin.