Human ST6 Gal Sialyltransferase 1/
ST6GAL1 Antibody
Human ST6 Gal Sialyltransferase 1/
ST6GAL1 Antibody Summary
Lys27-Cys406
Accession # P15907
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ST6 Gal Sialyltransferase 1/ST6GAL1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for ST6GAL1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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ST6 Gal Sialyltransferase 1/ST6GAL1 in Human Liver. ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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ST6 Gal Sialyltransferase 1/ST6GAL1 in Human Prostate. ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human ST6 Gal Sialyltransferase 1/ST6GAL1 by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of ST6 Gal Sialyltransferase 1/ST6GAL1 in U266 human myeloma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative). ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed U266 human myeloma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative) using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST6 Gal Sialyltransferase 1/ST6GAL1
Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).
- Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
- Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
- Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
- Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
- Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
- Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
- Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
- Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
- Kitazume, S. et al. (2009) Glycobiology 19:479.
Product Datasheets
Citations for Human ST6 Gal Sialyltransferase 1/
ST6GAL1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface
Authors: X Zou, J Lu, Y Deng, Q Liu, X Yan, Y Cui, X Xiao, M Fang, F Yang, H Sawaki, T Sato, B Tan, X Lu, B Feng, A Kuno, H Narimatsu, C Gao, Y Zhang
Oncogene, 2022;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees
Authors: J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID-
EBioMedicine, 2022;87(0):104408.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness
Authors: NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau
Cancer Gene Therapy, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sialyltransferase ST6GAL-1 mediates resistance to chemoradiation in rectal cancer
Authors: M Smithson, R Irwin, G Williams, KL Alexander, LE Smythies, M Nearing, MC McLeod, S Al Diffalh, SL Bellis, KM Hardiman
The Journal of Biological Chemistry, 2022;0(0):101594.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover
Authors: KL Alexander, CA Serrano, A Chakrabort, M Nearing, LN Council, A Riquelme, M Garrido, SL Bellis, LE Smythies, PD Smith
J. Biol. Chem., 2020;0(0):.
Species: Human
Sample Types: Organoid
Applications: ICC -
Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance
Authors: L Ou, X He, N Liu, Y Song, J Li, L Gao, X Huang, Z Deng, X Wang, S Lin
Mol Med Rep, 2020;21(3):1449-1460.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glycosyltransferase ST6Gal-I promotes the epithelial to mesenchymal transition in pancreatic cancer cells
Authors: CM Britain, N Bhalerao, AD Silva, A Chakrabort, DJ Buchsbaum, MR Crowley, DK Crossman, YJK Edwards, SL Bellis
J Biol Chem, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Regulation of inflammatory signaling by the ST6Gal-I sialyltransferase
Authors: AT Holdbrooks, KE Ankenbauer, J Hwang, SL Bellis
PLoS ONE, 2020;15(11):e0241850.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Sox2 promotes expression of the ST6Gal-I glycosyltransferase in ovarian cancer cells
Authors: KA Dorsett, RB Jones, KE Ankenbauer, AB Hjelmeland, SL Bellis
J Ovarian Res, 2019;12(1):93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transfer of Functional Cargo in Exomeres
Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
Cell Rep, 2019;0(0):.
Species: Human
Sample Types: Exosomes
Applications: Western Blot -
Glycoengineering design options for IgG1 in CHO cells using precise gene editing
Authors: MA Schulz, W Tian, Y Mao, J Van Coilli, L Sun, JS Larsen, YH Chen, C Kristensen, SY Vakhrushev, H Clausen, Z Yang
Glycobiology, 2018;0(0):.
Species: Hamster
Sample Types: Whole Cells
Applications: ICC -
Unravelling the Role of O-glycans in Influenza A Virus Infection
Authors: J Mayr, K Lau, JCC Lai, IA Gagarinov, Y Shi, S McAtamney, RWY Chan, J Nicholls, M von Itzste, T Haselhorst
Sci Rep, 2018;8(1):16382.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death
Authors: CM Britain, AT Holdbrooks, JC Anderson, CD Willey, SL Bellis
J Ovarian Res, 2018;11(1):12.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling
Authors: RB Jones, KA Dorsett, AB Hjelmeland, SL Bellis
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
Authors: AT Holdbrooks, CM Britain, SL Bellis
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
B-cell-independent sialylation of IgG
Authors: Mark B Jones
Proc Natl Acad Sci USA, 2016;113(26):7207-12.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype
Cancer Res, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans.
Authors: Suzuki O, Abe M
Int J Oncol, 2014;44(5):1433-42.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines.
Authors: Swindall A, Londono-Joshi A, Schultz M, Fineberg N, Buchsbaum D, Bellis S
Cancer Res, 2013;73(7):2368-78.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
ST6Gal-I sialyltransferase confers cisplatin resistance in ovarian tumor cells.
Authors: Schultz, Matthew, Swindall, Amanda F, Wright, John W, Sztul, Elizabet, Landen, Charles, Bellis, Susan L
J Ovarian Res, 2013;6(1):25.
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ST6GAL1 Antibody
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ST6GAL1 Antibody?
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Used this Ab for both Western and immunofluorescence on mouse ST6GAL1 transfected CHO cells. Fixed cells with 4% Paraformaldehyde and permeabilized with 0.5% saponin.
