Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody

  (9 citations)
(1 Review)
    
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Product Details
Citations (9)
FAQs
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  • Species Reactivity
    Human
  • Specificity
    Detects human Gal Sialyltransferase 1/ST6GAL1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Gal Sialyltransferase 1/ST6GAL1
    Lys27-Cys406
    Accession # P15907
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human ST6GAL1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ST6GAL1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human ST6 Gal Sialyltransferase 1/ST6GAL1 by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST6 Gal Sialyltransferase 1/ST6GAL1

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

  • References:
    1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
    2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
    3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
    4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
    5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
    6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
    7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
    8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
    9. Kitazume, S. et al. (2009) Glycobiology 19:479.
  • Long Name:
    ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1
  • Entrez Gene IDs:
    6480 (Human); 20440 (Mouse); 25197 (Rat)
  • Alternate Names:
    Alpha 2,6-ST; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CD75; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; EC 2.4.99.1; MGC48859; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1; SIAT1; SIAT1Alpha 2,6-ST 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Applications
Sample Type
  1. The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling
    Authors: RB Jones, KA Dorsett, AB Hjelmeland, SL Bellis
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  2. Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death
    Authors: CM Britain, AT Holdbrooks, JC Anderson, CD Willey, SL Bellis
    J Ovarian Res, 2018;11(1):12.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
    Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  4. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
    Authors: AT Holdbrooks, CM Britain, SL Bellis
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. B-cell-independent sialylation of IgG
    Authors: Mark B Jones
    Proc Natl Acad Sci USA, 2016;113(26):7207-12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  6. The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans.
    Authors: Suzuki O, Abe M
    Int J Oncol, 2014;44(5):1433-42.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines.
    Authors: Swindall A, Londono-Joshi A, Schultz M, Fineberg N, Buchsbaum D, Bellis S
    Cancer Res, 2013;73(7):2368-78.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  9. ST6Gal-I sialyltransferase confers cisplatin resistance in ovarian tumor cells.
    Authors: Schultz, Matthew, Swindall, Amanda F, Wright, John W, Sztul, Elizabet, Landen, Charles, Bellis, Susan L
    J Ovarian Res, 2013;6(1):25.

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 210  
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody AF5924 Array AF5924
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 ICC/IF Mouse Anonymous 03/21/2018
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Immunocytochemistry/Immunofluorescence Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody AF5924
Immunocytochemistry/Immunofluorescence: Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antibody [AF5924].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested ST6GAL1 transfected CHO cells
Species Mouse

Other Experimental Details

Other Experimental Details Used this Ab for both Western and immunofluorescence on mouse ST6GAL1 transfected CHO cells. Fixed cells with 4% Paraformaldehyde and permeabilized with 0.5% saponin.

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