Stabilin‑1 in Human Liver. Stabilin‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stabilin‑1 Monoclonal Antibody (Catalog # MAB3825) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor-1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor-1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrin-coated pits (CGAs). Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SI-CLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin-1 that diverges after aa 746 has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.
Entrez Gene IDs:
23166 (Human); 192187 (Mouse); 290559 (Rat)
CLEVER-1; fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-1; Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 1; FEEL1; FEEL-1; FEEL-1MS-1 antigen; FELE-1; FEX1; KIAA0246common lymphatic endothelial and vascular endothelial receptor-1; MS-1 Antigen; STAB1; STAB-1; stabilin 1; Stabilin1; Stabilin-1
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