|Stabilin‑1 in Human Liver. Stabilin‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stabilin‑1 Monoclonal Antibody (Catalog # MAB3825) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor-1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor-1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrin-coated pits (CGAs). Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SI-CLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin-1 that diverges after aa 746 has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.
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