Human SVEP1 Antibody

Catalog # Availability Size / Price Qty
MAB9774-100
MAB9774-SP
SVEP1 in MG‑63 Human Cell Line.
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Human SVEP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SVEP1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 982903
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human SVEP1 peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry SVEP1 antibody in MG-63 Human Cell Line by Immunocytochemistry (ICC). View Larger

SVEP1 in MG‑63 Human Cell Line. SVEP1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry SVEP1 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

SVEP1 in Human Placenta. SVEP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SVEP1

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Long Name
Sushi, Von Willebrand Factor Type A, EGF And Pentraxin Domain Containing 1
Entrez Gene IDs
79987 (Human); 64817 (Mouse); 685899 (Rat)
Alternate Names
C9orf13; CCP module-containing protein 22; CCP22; FLJ16013; FLJ90719; POLYDOM; selectin-like protein; SELOB; SEL-OB; sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1; sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1; SVEP1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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