Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 959904
View all Synaptophysin Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Synaptophysin
Glu50-Phe106
Accession # P08247

Specificity

Detects human Synaptophysin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Synaptophysin Antibody

Synaptophysin antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

Synaptophysin in SH‑SY5Y Human Cell Line.

Synaptophysin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Synaptophysin Monoclonal Antibody (Catalog # MAB5555) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Synaptophysin antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Synaptophysin in Mouse Brain.

Synaptophysin was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Mouse Anti-Human Synaptophysin Monoclonal Antibody (Catalog # MAB5555) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human Synaptophysin Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Synaptophysin

Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic
C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin.

Alternate Names

SYP

Entrez Gene IDs

6855 (Human)

Gene Symbol

SYP

UniProt

P08247

Additional Synaptophysin Products

Product Documents for Human Synaptophysin Antibody

Certificate of Analysis

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Product Specific Notices for Human Synaptophysin Antibody

For research use only

Citations for Human Synaptophysin Antibody

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Protocols

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