Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 761811
View all Syntaxin 1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 1A
Met1-Leu165
Accession # Q16623

Specificity

Detects human Syntaxin 1A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Epimorphin/Syntaxin 2 is observed, 25% cross-reactivity with rhSyntaxin 1B2 and rhSyntaxin 3 is observed, and no cross-reactivity with rhSyntaxin 1B1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Syntaxin 1A Antibody

Detection of Human and Rat Syntaxin 1A antibody by Western Blot.

Detection of Human and Rat Syntaxin 1A by Western Blot.

Western blot shows lysates of human brain tissue and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Syntaxin 1A Monoclonal Antibody (Catalog # MAB7237) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Syntaxin 1A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 1A antibody in Human Brain by Immunohistochemistry (IHC-P).

Syntaxin 1A in Human Brain.

Syntaxin 1A was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Syntaxin 1A Monoclonal Antibody (Catalog # MAB7237) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Syntaxin 1A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Western Blot

0.1 µg/mL
Sample: Human brain tissue and rat brain tissue

Reviewed Applications

Read 1 review rated 5 using MAB7237 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin 1A

STX1A (Syntaxin [Greek for 'organizing']1A; also HPC-1) is a 34-36 kDa member of the syntaxin family of proteins. It is a t-SNARE that is widely expressed in neurons, and is involved in the exocytosis of neurotransmitters at the presynaptic membrane. STX1A is transported intracellularly by microtubule-associated syntabulin, and its availability appears to be regulated through binding to LGI3. When released from LGI3, STX1A interacts with SNAP25 and VAMP2 to form the SNARE complex involved in exocytotic vesicle release. Human STX1A is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 288 amino acids (aa) in length. It contains a 265 aa N-terminal cytoplasmic domain that contains one coiled‑coil region (aa 68-109), a t-SNARE domain with a coiled‑coil region (aa 192-254), and a C-terminal transmembrane sequence (aa 266-286). There are three potential isoform variants. One shows a 34 aa substitution for aa 227‑288, while another termed STX1C is likely soluble, and contains a 25 aa substitution for the same aa sequence above encompassing aa 227‑288. A third variant shows a five aa substitution for aa 1-10. Over aa 1-165, human STX1A shares 99% aa sequence identity with mouse STX1A.

Alternate Names

Brain Syntaxin, HPC-1, p35-1, STX1A, SYN1A

Entrez Gene IDs

6804 (Human); 20907 (Mouse); 116470 (Rat)

Gene Symbol

STX1A

UniProt

Q16623 (Human)

Additional Syntaxin 1A Products

Product Documents for Human Syntaxin 1A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Syntaxin 1A Antibody

For research use only

Customer Reviews for Human Syntaxin 1A Antibody (1)

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  • Human Syntaxin 1A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 03/03/2022
    Human Syntaxin 1A Antibody MAB7237

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