Human TFEB Antibody

Catalog # Availability Size / Price Qty
MAB9170-SP
MAB9170-100
Detection  of Human TFEB by Western Blot.
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Human TFEB Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFEB in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 954604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human TFEB synthetic peptide
Pro384-Ala446
Accession # P19484
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human TFEB by Western Blot. View Larger

Detection of Human TFEB by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TFEB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TFEB  in A549 Human Cell Line. View Larger

TFEB in A549 Human Cell Line. TFEB was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFEB

Transcription Factor EB/TFEB binds E-box (5'-CANNTG-3') where it plays a role in T cell activation through expression of CD40L and CLEAR-box (5'-GTCACGTGAC-3') sequences where it promotes expression of lysosomal genes essential for regulation of autophagy.  TFEB is localized to the cytoplasm and translocates to the nucleus under aberrant lysosomal storage conditions. Phosphorylation of S211 in an MTORC1 dependent manner sequesters TFEB in the cytoplasm through association with 14-3-3 family proteins. Inhibition of MTORC1 causes dissociation from this complex and rapid accumulation in the nucleus.

Long Name
Transcription Factor EB
Entrez Gene IDs
7942 (Human); 21425 (Mouse); 316214 (Rat)
Alternate Names
ALPHATFEB; BHLHE35; Class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; TCFEBbHLHe35; TFEB; transcription factor EB

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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