Human TFEB Antibody

Catalog # Availability Size / Price Qty
Detection of Human TFEB by Western Blot.
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Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human TFEB Antibody Summary

Species Reactivity
Detects human TFEB in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 954604
Protein A or G purified from hybridoma culture supernatant
Human TFEB synthetic peptide
Accession # P19484
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TFEB antibody by Western Blot. View Larger

Detection of Human TFEB by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TFEB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TFEB antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

TFEB in A549 Human Cell Line. TFEB was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFEB

Transcription Factor EB/TFEB binds E-box (5'-CANNTG-3') where it plays a role in T cell activation through expression of CD40L and CLEAR-box (5'-GTCACGTGAC-3') sequences where it promotes expression of lysosomal genes essential for regulation of autophagy.  TFEB is localized to the cytoplasm and translocates to the nucleus under aberrant lysosomal storage conditions. Phosphorylation of S211 in an MTORC1 dependent manner sequesters TFEB in the cytoplasm through association with 14-3-3 family proteins. Inhibition of MTORC1 causes dissociation from this complex and rapid accumulation in the nucleus.

Long Name
Transcription Factor EB
Entrez Gene IDs
7942 (Human); 21425 (Mouse); 316214 (Rat)
Alternate Names
ALPHATFEB; BHLHE35; Class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; TCFEBbHLHe35; TFEB; transcription factor EB

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Citations for Human TFEB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Formononetin alleviates hepatic steatosis by facilitating TFEB-mediated lysosome biogenesis and lipophagy
    Authors: Y Wang, H Zhao, X Li, Q Wang, M Yan, H Zhang, T Zhao, N Zhang, P Zhang, L Peng, P Li
    J. Nutr. Biochem., 2019;73(0):108214.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. IFN? Impairs Autophagic Degradation of mtDNA Promoting Autoreactivity of SLE Monocytes in a STING-Dependent Fashion
    Authors: K Gkirtziman, E Kabrani, D Nikoleri, A Polyzos, A Blanas, P Sidiropoul, A Makrigiann, G Bertsias, DT Boumpas, P Verginis
    Cell Rep, 2018;25(4):921-933.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Human TFEB Antibody
By Anonymous on 02/11/2022
Application: ICC/IF Sample Tested: A549 Human Cell Line Species: Human