Human TLR10 Antibody

Catalog # Availability Size / Price Qty
MAB6619
MAB6619-SP
Human TLR10 Antibody in Data
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Product Details
Citations (1)
FAQs
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Human TLR10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 5, 7, 8, 9, recombinant mouse (rm) TLR6, 11, 12, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhTIGIRR, rhMD-1, rhMD-2, rhTIRAP, or rmRP105 is observed.
Source
Monoclonal Mouse IgG2B Clone # 670719
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TLR10
Asn168-Lys383
Accession # Q9BXR5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TLR10 antibody by Western Blot. View Larger

Detection of Human TLR10 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TLR10 Monoclonal Antibody (Catalog # MAB6619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLR10 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR10

Toll-like receptor 10 (TLR10, CD290) is a 91-100 kDa member of the Toll-like receptor family. Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence and a 792 aa mature chain. The mature chain constitutes a single-pass type I transmembrane protein. The extracellular domain (ECD) extends from aa 20-576 and the cytoplasmic region consists of aa 598-811. Human TLR10 contains 15 leucine-rich repeats plus eight potential sites for N-linked glycosylation. In addition, it contains one cytoplasmic TIR domain. Functionally, TLR10 is involved in the innate immune response to microbial agents. Specifically, it acts via MYD88 and TRAF6, which leads to NF-kappa-B activation, cytokine secretion, and an inflammatory response. Human TLR10 shares 71% aa sequence identity with rat TLR10.

Long Name
Toll-like Receptor 10
Entrez Gene IDs
81793 (Human)
Alternate Names
CD290 antigen; CD290; MGC104967; MGC126398; MGC126399; TLR10; toll-like receptor 10

Product Datasheets

Citation for Human TLR10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Toll-like receptor 10 (TLR10) exhibits suppressive effects on inflammation of prostate epithelial cells
    Authors: Y Fan, L Yang, Q Wei, Y Ding, Z Tang, P Tan, T Lin, D Guo, S Qiu
    Asian J. Androl., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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