Human TLR4 Antibody (285227)

Name: Human TLR4 Antibody (285227)
Brand: R&D Systems
SKU: MAB1478
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # MAB1478

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Citations (6)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 285227

View all TLR4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206

Specificity

Detects human TLR4 in direct ELISAs and Western blots. Shows approximately 25% cross‑reactivity with recombinant mouse (rm) TLR4 and no cross-reactivity with recombinant human TLR1, 2, 3, or rmTLR6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human TLR4 Antibody (285227)

Detection of TLR4 by Western Blot

PNS attenuated fibrosis associated with TLR 4 pathway in HFD-fed mice but not ob/ob mice. a Representative Sirius Red staining examination in liver sections of ob/ob and HFD mice treated with PNS or vehicle (Scale bars, × 50 μm). b, d The mRNA expression of fibrosis genes, including Collegen I, Collegen IV and alpha -SMA in HFD and ob/ob mice treated with PNS or vehicle. c, e The mRNA levels of inflammatory gens (TNF-alpha, IL-6 and CD14) in HFD and ob/ob mice treated with PNS or vehicle. f Immunofluorescence staining of alpha -SMA protein (red) staining in HFD and ob/ob mice treated with PNS or vehicle (Scale bars, × 20 μm). g–j The expression of TLR4 and phosphorylated p-38 proteins in HFD and ob/ob mice were evaluated by the western blotting analysis Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33656663), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TLR4 Antibody (285227)

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TLR4/MD‑2 Complex (Catalog # 3146-TM)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. MD-2, also known as ESOP-1, is a 25 kDa secreted protein that is required for TLR4-mediated responses to bacterial lipopolysaccharide (LPS) (1-3). The human TLR4 cDNA encodes an 839 amino acid (aa) precursor that contains a 23 aa signal sequence, a 608 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other TLRs and 60-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. The human MD‑2 cDNA encodes a 160 aa precursor with an 18 aa signal sequence (5). Human MD-2 shares 20% aa sequence identity with MD-1 and 62-64% aa sequence identity with bovine, mouse, and rat MD-2. MD-2 associates with TLR4 on monocytes, macrophages, dendritic cells, and B cells (5-7). MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (7, 8). MD-2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).