Human TLR4 Antibody

Catalog # Availability Size / Price Qty
MAB14783
MAB14783-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human TLR4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 10, recombinant mouse (rm) TLR4, rmTLR6, rhIL-1RI, rhIL-1RII, rhIL-1RAcP, rhST2, rhIL-18 R alpha, rhIL-1Rrp2, rhIL-18 R beta  , rhSIGIRR, rhTIGIRR, rhMD-1, rhMD‑2, and rmRP105 is observed.
Source
Monoclonal Mouse IgG2B Clone # 610017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. MD-2, also known as ESOP-1, is a 25 kDa secreted protein that is required for TLR4-mediated responses to bacterial lipopolysaccharide (LPS) (1-3). The human TLR4 cDNA encodes an 839 amino acid (aa) precursor that contains a 23 aa signal sequence, a 608 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. The human MD-2 cDNA encodes a 160 aa precursor with an 18 aa signal sequence (5). Human MD-2 shares 20% aa sequence identity with MD-1 and 62%-64% aa sequence identity with bovine, mouse, and rat MD-2. MD-2 associates with TLR4 on monocytes, macrophages, dendritic cells, and B cells (5-7). MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (7, 8). MD-2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

References
  1. Gangloff, M. and N.J. Gay (2004) Trends Biochem. Sci. 29:294.
  2. Palsson-McDermott, E.M. and L.A. O’Neill (2004) Immunology 113:153.
  3. Miyake, K. (2004) Semin. Immunol. 16:11.
  4. Medzhitov, R. et al. (1997) Nature 388:394.
  5. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  6. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
  7. Akashi, S. et al. (2000) J. Immunol. 164:3471.
  8. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
  9. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
  10. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
  11. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4186.
  12. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
  13. Pugin, J. et al. (2004) Blood 104:4071.
Long Name
Toll-like Receptor 4
Entrez Gene IDs
7099 (Human); 21898 (Mouse); 29260 (Rat)
Alternate Names
ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

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Citations for Human TLR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Intratumoral G100, a TLR4 agonist, induces anti-tumor immune responses and tumor regression in patients with Merkel cell carcinoma
    Authors: Shailender Bhatia, Natalie J. Miller, Hailing Lu, Natalie V. Vandeven, Dafina Ibrani, Michi M. Shinohara et al.
    Clinical Cancer Research
  2. Placental inflammation by HMGB1 activation of TLR4 at the syncytium
    Authors: Line H. Tangerås, Gabriela B. Silva, Guro S. Stødle, Lobke M. Gierman, Bente Skei, Karin Collett et al.
    Placenta

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