Human TR4/NR2C2 Antibody

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Detection of Human TR4/NR2C2 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (3)
Supplemental Products

Human TR4/NR2C2 Antibody Summary

Species Reactivity
This antibody specifically recognizes human TR4 and cross-reacts with mouse and rat TR4. Not yet tested in other species.
Monoclonal Mouse IgG2A Clone # H0107B
Protein A or G purified from hybridoma culture supernatant
Recombinant human TR4/NR2C2
A liquid formulation in physiologic saline with 0.1% NaN3.


Recommended Concentration
Western Blot
This antibody can be used at 2 μg/mL with the appropriatesecondary reagents to detect human TR4.
This antibody can be used at 10 μg/mL with theappropriate secondary reagents to detect human TR4.
Optimal dilutions should be determined by eachlaboratory.
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriatesecondary reagents to detect human TR4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Human TR4/NR2C2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human TR4/NR2C2 by Immunocytochemistry/Immunofluorescence IHC staining result investigating TR4 level and macrophage infiltration in tumor tissues of PCa patients, as well as TIMP-1/MMP2/MMP9 signaling. IHC staining was performed using TR4 antibody (1:300),CD68 antibody (1:200),TIMP-1 antibody (1:200),MMP2 antibody (1:200),MMP9 antibody (1:200). Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human TR4/NR2C2 by Western Blot View Larger

Detection of Human TR4/NR2C2 by Western Blot Knocking down TR4 reduces macrophage recruitment to PCa cells in co-culture system using Transwell assays. A. The illustration of macrophage recruitment (migration) and PCa invasion (invasion) in vitro model. The PCa cells were placed on the upper chamber and THP-1 cells in the lower chamber, co-cultured for 24 hours and then four kinds of conditioned media (CM) were collected: PCa scr/THP-1 scr, PCa siTR4/THP-1 scr, PCa scr/THP-1 siTR4, PCa siTR4/THP-1 siTR4. The CMs were diluted with 10% FBS RPMI media at 1:1 ratio, then put into the lower chamber of other transwell plates for macrophage migration assays and PCa cells invasion assays. B. The manipulation of TR4 in PCa cells and THP-1 cells. Western blotting to show the knock-down efficiency of TR4 in C4-2 and CWR22Rv-1 PCa cell lines and THP-1 macrophage cell line. C-E. Macrophage recruitment under different CMs. Upper are CM from C4-2/THP-1 cells and lower are from CWR22Rv1/THP-1 cells. Knock-down of TR4 in both PCa and THP-1 cell lines is shown in the left panels, knock-down of TR4 in PCa cells alone shown as D and knock-down of TR4 in THP-1 cells alone shown in as E. Note: * P < 0.05; ** P < 0.01; ns P > 0.05. Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: TR4/NR2C2

Human Testicular Receptor 4 (TR4, TAK1; NR2C2) is a member of the Orphan Nuclear Receptor family. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9 kb mRNA in many tissues and as a 2.8 kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4 alpha 1 and TR4 alpha 2 that differ in 19 amino acids coded by two separate exons. Both products translated from the 9 kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic acid-mediated transactivation.

Long Name
Testicular Orphan Nuclear Receptor 4
Entrez Gene IDs
7182 (Human)
Alternate Names
hTAK1; NR2C2; Nuclear hormone receptor TR4; nuclear receptor subfamily 2 group C member 2; nuclear receptor subfamily 2, group C, member 2; Orphan nuclear receptor TAK1; Orphan nuclear receptor TR4; TAK1; TAK1TR4TR2R1; Testicular receptor 4; TR4 nuclear hormone receptor; TR4

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Citations for Human TR4/NR2C2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Anti-recombination function of MutS alpha restricts telomere extension by ALT-associated homology-directed repair
    Authors: Barroso-GonzAlez J, GarcIa-ExpOsito L, Galaviz P et al.
    Cell reports
  2. TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres
    Authors: Porreca RM, Herrera-Moyano E, Skourti E et al.
  3. Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signals.
    Authors: Ding X, Yang D, Xia L, Chen B, Yu S, Niu Y, Wang M, Li G, Chang C
    Mol Cancer, 2015-01-27;14(0):16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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