Human TR alpha/NR1A1 Antibody (H2804)

R&D Systems | Catalog # PP-H2804-00

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H2804
View all TR alpha/NR1A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human TR alpha/NR1A1
aa 2-51

Specificity

This antibody specifically recognizes human TR alpha and cross-reacts with mouse and rat TR alpha. The antibody does not cross-react with human TR beta. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TR alpha/NR1A1 Antibody (H2804)

Detection of TR alpha /NR1A1 by Western Blot

Detection of TR alpha /NR1A1 by Western Blot

Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR beta silencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha /THRA, thyroid hormone receptor alpha; TR beta /THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TR alpha /NR1A1 by Western Blot

Detection of TR alpha /NR1A1 by Western Blot

Progestins enhanced the growth of THRB-silenced EC cells. (A) THRA or THRB silencing efficiency in RL95-2 and KLE cells. (B,C) Protein expression of TR alpha or TR beta silencing in RL95-2 and KLE cells. (D,G) Cell viability after silencing THRA or THRB in RL95-2 and KLE cells. The cells were treated with si-THRA or si-THRB for 48 h and then cultured in fresh media for 24, 48, 72, and 96 h. (E,F) RL95-2 or (H,I) KLE cells were pretreated with si-THRA or si-THRB for 48 h and then treated with 30 μM MPA (E,H) or NOMAc (F,I); meanwhile, 100 nM T3 was added for 48 h to examine cell viability. The cell viability was normalized to the control, which was set at 100%. The results are presented as mean ± SEM of three independent experiments. TR alpha /THRA, thyroid hormone receptor alpha; TR beta /THRB: thyroid hormone receptor beta; si-Ctrl, negative control treated with siRNA solvent; si-THRA, silenced THRA; si-THRB, silenced THRB. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36293372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TR alpha/NR1A1 Antibody (H2804)

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect human TR alpha.

Immunohistochemistry

This antibody can be used at 20 - 50 μg/mL with the appropriate secondary reagents to detect human TR alpha.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions and at 3 μg/mL under non-reducing conditions with the appropriate secondary reagents to detect human TR alpha.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: TR alpha/NR1A1

Thyroid Hormone Receptor alpha (TR alpha, THRA; NR1A1) is a member of the Nuclear Hormone Receptor superfamily. The ligands for TR alpha are the thyroid hormones, which exist in two forms: T4 and T3. TR alpha plays a critical role in the differentiation, growth, metabolism and physiology of a wide variety of tissues. The major partners of TRs are the Retinoid X Receptors (RXRs), which strongly enhance their ability to bind to specific DNA sequences and contribute to the specificity of TR.

Long Name

Thyroid Hormone Receptor alpha

Alternate Names

NR1A1, THRA, v-ErbA1

Entrez Gene IDs

7067 (Human)

Gene Symbol

THRA

Additional TR alpha/NR1A1 Products

Product Documents for Human TR alpha/NR1A1 Antibody (H2804)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TR alpha/NR1A1 Antibody (H2804)

For research use only

Citations for Human TR alpha/NR1A1 Antibody (H2804)

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Protocols

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