Human Troponin I/TNNI3 Antibody
Human Troponin I/TNNI3 Antibody Summary
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85941).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Troponin I/TNNI3 ELISA Standard Curve. Recombinant Human Troponin I/TNNI3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody was biotinylated (Catalog # MAB85941) and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Troponin I/TNNI3
Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.
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