Human Troponin I/TNNI3 Antibody

Catalog # Availability Size / Price Qty
MAB85942-100
MAB85942-SP

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Human Troponin I/TNNI3 ELISA Standard Curve.
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Human Troponin I/TNNI3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Troponin I/TNNI3 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 923952
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Troponin I/TNNI3
Met1-Ser210
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85941).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Troponin I/TNNI3 ELISA Standard Curve. Recombinant Human Troponin I/TNNI3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody was biotinylated (Catalog # MAB85941) and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin I/TNNI3

Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Long Name
Troponin I3, Cardiac Type
Entrez Gene IDs
7137 (Human)
Alternate Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; CTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1; TNNC1troponin I, cardiac; TNNI3,; troponin I type 3 (cardiac); troponin I, cardiac muscle; Troponin I/TNNI3

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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