Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 730804
View all VISTA/B7-H5/PD-1H Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H
Phe33-Ala194
Accession # Q9H7M9

Specificity

Detects human VISTA/B7-H5/PD-1H in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse VISTA/B7-H5/PD-1H is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VISTA/B7-H5/PD-1H Antibody

Detection of VISTA/B7-H5/PD-1H antibody in Human PBMCs antibody by Flow Cytometry.

Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) or (B) Mouse IgG2BIsotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P). View our protocol for Staining Membrane-associated Proteins.
VISTA/B7-H5/PD-1H antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

VISTA/B7-H5/PD-1H in BG01V Human Embryonic Stem Cells.

VISTA/B7-H5/PD-1H was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of VISTA/B7-H5/PD-1H by Flow Cytometry

Detection of VISTA/B7-H5/PD-1H by Flow Cytometry

VISTA expression in human monocytes. (A) Scheme showing the surface expression of VISTA and proteases involved in shedding and also demonstrating the action of inhibitors. (B) Monocytes express VISTA on the Surface (histogram of VISTA expression on monocytes) and (C) release soluble VISTA via protease cleavage. VISTA release mediated by cleavage is reduced in the presence of matrix metalloproteinase inhibitors. Data are the mean values ± SEM (n=6). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35634346), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of VISTA/B7-H5/PD-1H by Flow Cytometry

Detection of VISTA/B7-H5/PD-1H by Flow Cytometry

VISTA expression in human monocytes. (A) Scheme showing the surface expression of VISTA and proteases involved in shedding and also demonstrating the action of inhibitors. (B) Monocytes express VISTA on the Surface (histogram of VISTA expression on monocytes) and (C) release soluble VISTA via protease cleavage. VISTA release mediated by cleavage is reduced in the presence of matrix metalloproteinase inhibitors. Data are the mean values ± SEM (n=6). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35634346), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VISTA/B7-H5/PD-1H Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Reviewed Applications

Read 1 review rated 5 using MAB71261 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

Q9H7M9 (Human)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Human VISTA/B7-H5/PD-1H Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VISTA/B7-H5/PD-1H Antibody

For research use only

Citations for Human VISTA/B7-H5/PD-1H Antibody

Customer Reviews for Human VISTA/B7-H5/PD-1H Antibody (1)

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  • Human VISTA/B7-H5/PD-1H Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 04/21/2016
    immunophenotyping by flow cytometry. The antibody worked exactly as described to identify VISTA+ cells

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Protocols

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