Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 342603
View all Vitronectin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived Vitronectin

Specificity

Detects human Vitronectin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant bovine vitronectin or recombinant mouse vitronectin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Vitronectin Antibody

Detection of Human Vitronectin antibody by Western Blot.

Detection of Human Vitronectin by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vitronectin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vitronectin antibody in Human Bladder by Immunohistochemistry (IHC-P).

Vitronectin in Human Bladder.

Vitronectin was detected in immersion fixed paraffin-embedded sections of human bladder using Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Vitronectin antibody by Simple WesternTM.

Detection of Human Vitronectin by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for Vitronectin at approximately 85 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Vitronectin Monoclonal Antibody (Catalog # MAB2349). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Vitronectin by Western Blot

Detection of Human Vitronectin by Western Blot

Vitronectin expression is modulated by PI3K/AKT axis.A. Displays the pseudo blot extracted from Simple Western experiments. There are four cell-lines- MDA-MB-231, MCF7, MDA-MB-468, HCC1599, were used to evaluate the signaling cascade relationship. Different protein expression levels were normalized with an averaged house-keeping GAPDH and beta -actin expression B. Compares vitronectin concentration levels in four BC cell lines. C. PI3K concentration levels in BC same cell-lines. D. compares AKT concentration and, E. P-AKT concentration levels in the same four BC cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33211735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Vitronectin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder and breast

Simple Western

5 µg/mL
Sample: Human serum

Western Blot

0.1 µg/mL
Sample: Human serum

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2349 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vitronectin

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha v beta 3) and thrombin. The 459 aa mature human Vitronectin shows 74% amino acid identity with mouse Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Entrez Gene IDs

7448 (Human); 22370 (Mouse); 507525 (Bovine)

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Human Vitronectin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Vitronectin Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Vitronectin Antibody

Customer Reviews for Human Vitronectin Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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  • Human Vitronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/30/2019
    Paired with AF2349
    Human Vitronectin Antibody MAB2349
  • Human Vitronectin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HC11 cells
    Species: Human
    Verified Customer | Posted 02/22/2016
    Human Vitronectin Antibody MAB2349

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Protocols

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