hh Human Wnt-10b Antibody MAB7196: R&D Systems

Human Wnt-10b Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Wnt-10b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Wnt-6 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 793127
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Wnt-10b
    Asn29-Lys389
    Accession # O00744
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Wnt‑10b by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line and MDA‑MB‑468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Wnt‑10b at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Wnt‑10b by Simple WesternTM. Simple Western lane view shows lysate of SW480 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Wnt‑10b at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

  • References:
    1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
    2. Katoh, M. (2008) Curr. Drug Targets 9:565.
    3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
    4. Swiss Prot Accession # O00744.
    5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
    6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
    7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
    8. Wright, W.S. et al. (2007) Diabetes 56:295.
    9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
    10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
    11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
    12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
    13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
    14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 10B
  • Entrez Gene IDs:
    7480 (Human); 22410 (Mouse)
  • Alternate Names:
    protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12
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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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