Human ZnT-8 Antibody

Catalog # Availability Size / Price Qty
MAB7936
MAB7936-SP
Human ZnT-8 Antibody in Data
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Product Details
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Reviews (3)

Human ZnT-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZnT‑8 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 815039
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ZnT‑8
Lys268-Pro359
Accession # Q8IWU4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry ZnT-8 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

ZnT‑8 in Human Pancreas. ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZnT-8 Monoclonal Antibody (Catalog # MAB7936) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of ZNT-8 antibody in PANC-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ZNT-8 in PANC-1 Human Cell Line by Flow Cytometry. PANC-1 human cell line was stained with Mouse Anti-Human ZNT-8 Monoclonal Antibody (Catalog # MAB7936, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZnT-8

ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta ‑cells and alpha -cells, B cells, and adipocytes and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta -cells, provides a necessary component for proper insulin processing and granule storage. Furthermore, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.

Long Name
Zinc Transporter 8
Entrez Gene IDs
169026 (Human); 239436 (Mouse); 299903 (Rat)
Alternate Names
SLC30A8; solute carrier family 30 (zinc transporter), member 8; Solute carrier family 30 member 8; zinc transporter 8; zinc transporter ZnT-8; ZnT8; ZnT-8; ZNT8ZnT-8

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ZnT-8 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
33.33%
4 Star
66.67%
3 Star
0%
2 Star
0%
1 Star
0%

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Human ZnT-8 Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human ZnT-8 Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human,Mouse

Human ZnT-8 Antibody
By Anonymous on 08/31/2018
Application: ICC/IF Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human