MERS-CoV Spike RBD Antibody (1038342)

R&D Systems | Catalog # MAB107071

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Key Product Details

Species Reactivity

MERS-CoV

Applications

Blockade of Receptor-ligand Interaction, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1038342
View all Spike RBD Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived MERS-CoV Spike RBD
Glu367-Tyr606
Accession # YP_007188579.1

Specificity

Detects MERS-CoV Spike RBD in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MERS-CoV Spike RBD Antibody (1038342)

MERS-Cov Spike S1 protein binding to CD26-transfected Human Cell Line is Blocked by MERS-Cov Spike RBD Antibody

In a functional flow cytometry test, Recombinant MERS-Cov Spike S1 Fc-tagged protein (10606-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD26 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-MERS-Cov Spike RBD Monoclonal Antibody (Catalog # MAB107071) but not by (B) Mouse IgG2B Isotype Control (MAB0041). Protein binding was detected with Mouse Anti-Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Spike RBD in MERS RBD transfected cells.

Spike RBD was detected in immersion fixed MERS RBD transfected cells using Mouse Anti-MERS-CoV Spike RBD Monoclonal Antibody (Catalog # MAB107071) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for MERS-CoV Spike RBD Antibody (1038342)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional flow cytometry test, 50 μg/mL of Mouse Anti-MERS-Cov Spike RBD Antibody(Catalog # MAB107071) will block the binding of Recombinant MERS-Cov Spike S1 Fc-tagged protein (Catalog # 10606-CV) to HEK293 human embryonic kidney cell line transfected with recombinant human CD26.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MERS RBD transfected cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike RBD

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), was first reported in Saudi Arabia in 2012 as a novel coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes MERS-CoV, HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Located within the S1 subunit is the receptor binding domain (RBD). The RBD is responsible for the binding of MERS-CoV to dipeptidyl peptidase IV (DPP4, also known as human CD26) (4). The RBD of MERS-CoV shares 24% and 21% amino acid sequence (aa) identity with SARS-CoV RBD and SARS-Cov2 RBD, respectively. The low aa sequence identity is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). The S1 subunit, especially the RBD region, of MERS-CoV was commonly targeted for vaccinations or antiviral therapies (5-7).

References

  1. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
  2. Ogimi, C. et al. (2020) J Pediatric Infect Dis Soc doi: 10.1093/jpids/piaa037.
  3. Li, Y. et al. (2019) Engineering. 5:940.
  4. Raj, V.S. et al. (2013) Nature 495:251.
  5. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
  6. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
  7. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_007188579.1

Additional Spike RBD Products

Product Documents for MERS-CoV Spike RBD Antibody (1038342)

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Product Specific Notices for MERS-CoV Spike RBD Antibody (1038342)

For research use only

Related Research Areas

Infectious Diseases

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