Mouse C-Reactive Protein/CRP Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse C‑Reactive Protein/CRP in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CRP and recombinant rat CRP is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse C‑Reactive Protein/CRP
    His20-Ser225
    Accession # Q91XB3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse liver
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse
C‑Reactive Protein/CRP by Western Blot.
Western blot shows lysate of mouse serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1829) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for C‑Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Reactive Protein/CRP

C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins (1). CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa (2, 3). There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated (1, 4, 5). In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked (6). Mature mouse CRP shares 74%, 71%, 79%, and 68% aa sequence identity with rat, human, hamster and guinea pig CRP, respectively. In human, CRP is induced in hepatocytes principally by IL-6 (1). In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1 (1, 7), with another pentraxin, SAP, being IL-6 inducible (7). CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an antiinflammatory agent (8).

  • References:
    1. Hirschfield, G.M. and M.B. Pepys (2003) Q.J. Med 96:793.
    2. Whitehead, A.S. et al. (1990) Biochem. J. 266:283.
    3. Ohnishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 156:814.
    4. Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
    5. Black, S. et al. (2004) J. Biol. Chem. 279:48487.
    6. Rassouli, M. et al. (1992) J. Biol. Chem. 267:2947.
    7. Ku, N.-O. and R.F. Mortensen (1993) Cytokine 5:319.
    8. Mold, C. et al. (2002) J. Immunol. 169:7019.
  • Entrez Gene IDs:
    1401 (Human); 12944 (Mouse); 25419 (Rat)
  • Alternate Names:
    C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
    J. Virol., 2012;86(12):6620-31.
    Species: Mouse
    Sample Type: Serum
    Application: WB
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