Mouse CD36/SR-B3 Antibody

(14 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
    Gly30-Lys439
    Accession # Q3UAI3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Mouse CD36/SR-B3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse CD36/SR‑B3 Antibody (Catalog # AF2519)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CD36/SR‑B3 Biotinylated Antibody (Catalog # BAF2519)
  • ELISA Standard
     
    Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF (Catalog # 2519-CD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

  • Long Name:
    Scavenger Receptor Class B, Member 3
  • Entrez Gene IDs:
    948 (Human); 12491 (Mouse)
  • Alternate Names:
    CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. 2Intestinal epithelial cell Caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels
    Authors: JP Otis, MC Shen, V Quinlivan, JL Anderson, SA Farber
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  2. CD36 is essential for endurance improvement, changes in�whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training
    Authors: MCC Manio, S Matsumura, D Masuda, K Inoue
    Physiol Rep, 2017;5(10):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Cell-surface CD36 in monocyte/macrophage contributes to phagocytosis during the resolution phase of ischemic stroke in mice
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
    Authors: R Biedro?, A Peru?, S Józefowski
    PLoS ONE, 2016;11(4):e0153558.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: Binding Assay
  5. Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice.
    Authors: Lee S, Eguchi A, Tsuzuki S, Matsumura S, Inoue K, Iwanaga T, Masuda D, Yamashita S, Fushiki T
    PLoS ONE, 2015;10(7):e0133412.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation.
    Authors: Samovski D, Sun J, Pietka T, Gross R, Eckel R, Su X, Stahl P, Abumrad N
    Diabetes, 2015;64(2):353-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
    Authors: Pietka, Terri A, Sulkin, Matthew, Kuda, Ondrej, Wang, Wei, Zhou, Dequan, Yamada, Kathryn, Yang, Kui, Su, Xiong, Gross, Richard, Nerbonne, Jeanne M, Efimov, Igor R, Abumrad, Nada A
    J Biol Chem, 2012;287(46):38901-12.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice.
    Authors: Nagashima M, Watanabe T, Terasaki M
    Diabetologia, 2011;54(10):2649-59.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors.
    Authors: Makinen PI, Lappalainen JP, Heinonen SE, Leppanen P, Lahteenvuo MT, Aarnio JV, Heikkila J, Turunen MP, Yla-Herttuala S
    Cardiovasc. Res., 2010;88(3):530-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
    Authors: Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, Yehuda-Shnaidman E, Lee C, Lau J, Kurtz TW, Chan JY
    J. Biol. Chem., 2010;285(12):9292-300.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages.
    Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y
    Atherosclerosis, 2009;204(2):405-11.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Transcriptional regulation of fatty acid translocase/CD36 expression by CCAAT/enhancer-binding protein alpha.
    Authors: Qiao L, Zou C, Shao P, Schaack J, Johnson PF, Shao J
    J. Biol. Chem., 2008;283(14):8788-95.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury.
    Authors: Kim E, Tolhurst AT, Qin LY, Chen XY, Febbraio M, Cho S
    J. Neurosci., 2008;28(18):4661-70.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007;120(0):3053-63.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
Expand to show all 14 Citations
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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