Mouse FGF R4 Antibody

Catalog # Availability Size / Price Qty
AF2265
AF2265-SP
Detection of Mouse FGF R4 by Western Blot.
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Product Details
Citations (2)
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Mouse FGF R4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FGF R4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) FGF R4 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) FGF R3 alpha (IIIb) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse FGF R4 by Western Blot. View Larger

Detection of Mouse FGF R4 by Western Blot. Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF R4 at approximately 89 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FGF R4 in Mouse Embryo. View Larger

FGF R4 in Mouse Embryo. FGF R4 was detected in immersion fixed frozen sections of mouse embryo (11.5 d.p.c.) using Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF R4

FGF R4 is a transmembrane receptor tyrosine kinase that binds several ligands of the FGF family. It is expressed in the early embryo, and its expression has been suggested to play an important role in the formation of mesoderm and posterior structure (1). Recent studies also indicated that FGF R4 may be used as a marker to distinguish neural stem cells from other precursor cells (2). The extracellular domain of mouse FGF R4 shares 95% and 89% amino acid sequence homology with its rat and human homologs, respectively.

References
  1. Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
  2. Cai, J. (2002) Dev. Biol. 251:221.
Long Name
Fibroblast Growth Factor Receptor 4
Entrez Gene IDs
2264 (Human); 14186 (Mouse)
Alternate Names
CD334 antigen; CD334; EC 2.7.10; EC 2.7.10.1; FGF R4; FGFR4; FGFR-4; fibroblast growth factor receptor 4; JTK2hydroxyaryl-protein kinase; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase

Product Datasheets

Citations for Mouse FGF R4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Spinal cord injury induces astroglial conversion towards neuronal lineage
    Mol Neurodegener, 2016;11(1):68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  2. FGF15/FGFR4 integrates growth factor signaling with hepatic bile acid metabolism and insulin action.
    Authors: Shin DJ, Osborne TF
    J. Biol. Chem., 2009;284(17):11110-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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