Mouse Frizzled-1 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-4, rmFrizzled-7, and rmFrizzled-8 is observed, and less than 1% cross-reactivity with rmFrizzled-3 and rmFrizzled-6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
    Gln72-His248 (Met122Ile)
    Accession # O70421
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Frizzled‑1 Fc Chimera (Catalog # 1120-FZ)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Frizzled‑1 in Embryonic Mouse Intestine. Frizzled‑1 was detected in immersion fixed frozen sections of embryonic mouse intestine (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1120) overnight at
4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-1

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises 642 amino acid residues and shares 95% identity with the human orthologue in the CRD. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3).

Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).

  • References:
    1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59. 
    2. Hsieh, J-C. (2004) Front. Biosci., 9:1333. 
    3. Xu, L. et al. (2001) Matrix Biol. 2:147. 
    4. Tamai, K. et al. (2000) Nature 407:530. 
    5. Pinson, K. et al. (2000) Nature 407:535.
    6. Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
    7. Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
    8. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
  • Entrez Gene IDs:
    8321 (Human); 14362 (Mouse)
  • Alternate Names:
    DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  2. Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation.
    Authors: Kanei-Ishii C, Nomura T, Takagi T, Watanabe N, Nakayama KI, Ishii S
    J. Biol. Chem., 2008;283(45):30540-8.
    Species: Rat
    Sample Type: Recombinant Protein
    Application: WB
  3. Frizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomers.
    Authors: Chacon MA, Varela-Nallar L, Inestrosa NC
    J. Cell. Physiol., 2008;217(1):215-27.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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