Mouse Galectin-1 Antibody

(10 citations)
(5 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Galectin-1
    Ala2-Glu135
    Accession # P16045
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Galectin‑1 (Catalog # 1245-GA)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
    • Mouse Galectin-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Galectin‑1 Antibody (Catalog # AF1245)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Galectin‑1 Biotinylated Antibody (Catalog # BAF1245)
  • ELISA Standard
     
    Recombinant Mouse Galectin-1 Protein (Catalog # 1245-GA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Galectin‑1 in Mouse Thymus. Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of Galectin‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

  • References:
    1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
    2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
    3. Hughes, R.C. (2001) Biochimie 83:667.
    4. R&D Systems’ Cytokine Bulletin, Summer (2002).
    5. Goldring, K. et al. (2002) J. Cell Science 115:355.
  • Entrez Gene IDs:
    3956 (Human); 16852 (Mouse)
  • Alternate Names:
    14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity.
    Authors: Baker G, Chockley P, Yadav V, Doherty R, Ritt M, Sivaramakrishnan S, Castro M, Lowenstein P
    Cancer Res, 2014;74(18):5079-90.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair.
    Authors: Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, Jaiswal J
    Nat Commun, 2014;5(0):5646.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
    Authors: Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E
    PLoS ONE, 2012;7(7):e41372.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  4. Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
    Authors: Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
    Am. J. Pathol., 2011;178(3):1201-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2011;116(2):217-26.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  6. Impaired spatial and contextual memory formation in galectin-1 deficient mice.
    Mol Brain, 2011;4(0):33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome.
    Authors: Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K
    Lab. Invest., 2009;89(2):178-95.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
    Authors: Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA
    Nat. Neurosci., 2007;10(6):712-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
    Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
    Am. J. Pathol., 2007;170(2):546-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  10. Galectin-1 supports survival of naive T cells without promoting cell proliferation.
    Authors: Endharti AT, Zhou YW, Nakashima I, Suzuki H
    Eur. J. Immunol., 2005;35(1):86-97.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: IP
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Average Rating: 5 (Based on 5 reviews)

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We have 5 reviews tested in 2 species: Human, Mouse.
We have 5 reviews tested in 3 applications: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse Galectin-1 Antibody AF1245
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Excellent
 WB Mouse Anonymous 08/17/2016
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Western Blot Mouse Galectin-1 Antibody AF1245
Western Blot: Mouse Galectin-1 Antibody [AF1245]

Summary

ApplicationWestern Blot
Sample TestedU-87 MG human glioblastoma/astrocytoma cell line
SpeciesMouse

Other Experimental Details

Other Experimental Details1:2000 dilution in TBS-Tween 20 + 5% BSA
Western Blot Mouse Galectin‑1 Antibody AF1245
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Excellent
 WB Human Anonymous 10/26/2015
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Western Blot Mouse Galectin‑1 Antibody AF1245
Western Blot: Mouse Galectin‑1 Antibody [AF1245]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe antibody is very specific at 1:1000 and also at 1:2500 dilutions.
Specificity: Specific
Sensitivity: Sensitive
Buffer: BSA
Dilution: 1:1000
Immunohistochemistry Mouse Galectin‑1 Antibody AF1245
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Excellent
 IHC Mouse Anonymous 10/26/2015
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Immunohistochemistry Mouse Galectin‑1 Antibody AF1245
Immunohistochemistry: Mouse Galectin‑1 Antibody [AF1245]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsThe antibody specifically detects the stromal galectin-1 in normal tissues.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245
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Excellent
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245
Immunocytochemistry/Immunofluorescence: Mouse Galectin‑1 Antibody [AF1245]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman fibroblast
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThese are HFF-2 cells that show the staining for this antibody.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS`
Dilution: 1:500
Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245
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Excellent
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Mouse Galectin‑1 Antibody AF1245
Immunocytochemistry/Immunofluorescence: Mouse Galectin‑1 Antibody [AF1245]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedCancer Cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsVery specific in detection of galectin-1 in cancer cells.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000

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