Mouse HGF R/c-MET Antibody

(19 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-MET
    Glu25-Asn929
    Accession # P16056
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.01 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527-ME)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 256-GF) to immobilized Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527‑ME) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
HGF R/c‑MET in HT‑29 and U937 Human Cell Lines. HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (left panel) and U937 human histiocytic lymphoma cell line (right panel) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
HGF R/c‑MET in Mouse Embryo. HGF R/c‑MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF R/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular  alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin  alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11-18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11-18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation,
over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.

  • References:
    1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
    2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
    4. Chan, A.M. et al. (1988) Oncogene 2:593.
    5. Lee, C.-C. and K.M. Yamada (1994) J. Biol. Chem. 269:19457.
    6. Lee, C.-C., et al. (1995) J. Biol. Chem. 270:507.
    7. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
    8. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
    9. Ponzetto, C. et al. (1994) Cell 77:261.
    10. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
    11. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
    12. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
    13. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
    14. Wang, X. et al. (2002) Mol. Cell 9:411.
    15. Trusolino, L. et al. (2001) Cell 107:643.
    16. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
    17. Conrotto, P. et al. (2004) Oncogene 23:5131.
    18. Follenzi, A. et al. (2000) Oncogene 19:3041.
    19. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
  • Long Name:
    Hepatocyte Growth Factor Receptor
  • Entrez Gene IDs:
    4233 (Human); 17295 (Mouse)
  • Alternate Names:
    AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Receptor Tyrosine Kinase Met Promotes Cell Survival via Kinase-Independent Maintenance of Integrin ?3?1
    Authors: Lia Tesfay
    Mol Biol Cell, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
    Authors: Rachel Barrow-McG
    Nat Commun, 2016;7(0):11942.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and Hearing
    J Neurosci, 2016;36(31):8200-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  4. MET is required for the recruitment of anti-tumoural neutrophils.
    Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
    Nature, 2015;522(7556):349-53.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Capture
  5. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
    Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
    PLoS ONE, 2015;10(5):e0125797.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  6. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
    Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
    Nat Commun, 2015;6(0):6776.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter.
    Authors: Wei W, Howard P, Macarak E
    BMC Urol, 2013;13(0):62.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
    Authors: Tesio M, Golan K, Corso S
    Blood, 2011;117(2):419-28.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
    Authors: Gordin M, Tesio M, Cohen S
    J. Immunol., 2010;185(4):2020-31.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis.
    Authors: Moransard M, Sawitzky M, Fontana A, Suter T
    Glia, 2010;58(5):559-71.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  11. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.
    Authors: Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE
    Am. J. Physiol., Cell Physiol., 2010;298(3):C465-76.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  12. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation.
    Authors: Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009;297(2):C238-52.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  13. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  14. Purification and characterization of mouse lacrimal gland epithelial cells and reconstruction of an acinarlike structure in three-dimensional culture.
    Authors: Ueda Y, Karasawa Y, Satoh Y, Nishikawa S, Imaki J, Ito M
    Invest. Ophthalmol. Vis. Sci., 2009;50(5):1978-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  15. Ets-1 triggers and orchestrates the malignant phenotype of mammary cancer cells within their matrix environment.
    Authors: Furlan A, Vercamer C, Desbiens X, Pourtier A
    J. Cell. Physiol., 2008;215(3):782-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  16. A selective small molecule inhibitor of c-Met, PHA-665752, reverses lung premalignancy induced by mutant K-ras.
    Authors: Yang Y, Wislez M, Fujimoto N, Prudkin L, Izzo JG, Uno F, Ji L, Hanna AE, Langley RR, Liu D, Johnson FM, Wistuba I, Kurie JM
    Mol. Cancer Ther., 2008;7(4):952-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  17. Gastrointestinal hormones cause rapid c-Met receptor down-regulation by a novel mechanism involving clathrin-mediated endocytosis and a lysosome-dependent mechanism.
    Authors: Hoffmann KM, Tapia JA, Berna MJ, Thill M, Braunschweig T, Mantey SA, Moody TW, Jensen RT
    J. Biol. Chem., 2006;281(49):37705-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  18. A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.
    Authors: Lai L, Zeff RA, Goldschneider I
    Blood, 2005;107(5):1776-84.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. Metastatic squamous cell carcinoma cells that overexpress c-Met exhibit enhanced angiogenesis factor expression, scattering and metastasis in response to hepatocyte growth factor.
    Authors: Dong G, Lee TL, Yeh NT, Geoghegan J, Van Waes C
    Oncogene, 2004;23(37):6199-208.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 19 Citations
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