Mouse IL-1 RI Antibody AF771: R&D Systems

Mouse IL-1 RI Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) IL-18 R is observed and 5% cross-reactivity with rmIL-18 R beta, recombinant human IL-1 RI, and recombinant rat IL-1 Rrp2 is observed and less than 1% cross-reactivity with rmIL-1 RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
    Leu20-Lys338
    Accession # P13504
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑1 RI Fc Chimera (Catalog # 771-MR)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse lymph node and thymus
  • Neutralization
    Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral­ization by Mouse IL‑1 RI Antibody. Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  • References:
    1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
  • Long Name:
    Interleukin 1 Receptor I
  • Entrez Gene IDs:
    3554 (Human); 16177 (Mouse)
  • Alternate Names:
    CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Spinal astrocytic activation contributes to mechanical allodynia in a rat model of cyclophosphamide-induced cystitis
    Mol Pain, 2016;12(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. Neutrophils mediate blood-spinal cord barrier disruption in demyelinating neuroinflammatory diseases.
    Authors: Aube B, Levesque S, Pare A, Chamma E, Kebir H, Gorina R, Lecuyer M, Alvarez J, De Koninck Y, Engelhardt B, Prat A, Cote D, Lacroix S
    J Immunol, 2014;193(5):2438-54.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice.
    Authors: Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M
    PLoS ONE, 2013;8(11):e81744.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012;209(6):1219-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Interleukin-1beta causes long-term potentiation deficiency in a mouse model of septic encephalopathy.
    Authors: Imamura Y, Wang H, Matsumoto N, Muroya T, Shimazaki J, Ogura H, Shimazu T
    Neuroscience, 2011;187(0):63.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet.
    Authors: Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL
    Endocrinology, 2010;151(4):1622-32.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
    Authors: O&amp;amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG
    J. Immunol., 2007;178(11):6886-93.
    Species: Mouse
    Sample Type: Peritoneal Lavage
    Application: ELISA Development
  8. IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons.
    Authors: Davis CN, Tabarean I, Gaidarova S, Behrens MM, Bartfai T
    J. Neurochem., 2006;98(5):1379-89.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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