Mouse IL-1 RI Antibody

Catalog # Availability Size / Price Qty
AF771
AF771-SP

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IL‑1 RI in Mouse Skin.
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Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (3)

Mouse IL-1 RI Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse IL-18 R and recombinant human IL-1 RI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
Leu20-Lys338
Accession # P13504
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑1 RI Fc Chimera (Catalog # 771-MR)
Immunohistochemistry
1-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-1 RI antibody in Mouse Skin by Immunohistochemistry (IHC-Fr). View Larger

IL‑1 RI in Mouse Skin. IL-1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 RI Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 RI Antibody. Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. Mouse IL-1 RI shares 63% amino acid sequence homology with human IL-1 RI in their extracellular domains.

An IL-1 receptor accessory protein (1) that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta  but not IL-1ra, was identified. This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor I
Entrez Gene IDs
3554 (Human); 16177 (Mouse)
Alternate Names
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

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Citations for Mouse IL-1 RI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Phosphorylation of alpha B-Crystallin Involves Interleukin-1 beta -Mediated Intracellular Retention in Retinal Müller Cells: A New Mechanism Underlying Fibrovascular Membrane Formation
    Authors: Yamamoto, T;Kase, S;Shinkai, A;Murata, M;Kikuchi, K;Wu, D;Kageyama, Y;Shinohara, M;Sasase, T;Ishida, S;
    Investigative ophthalmology & visual science
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Reactive Bergmann glia play a central role in spinocerebellar ataxia inflammation via the JNK pathway
    Authors: Edamakanti, CR;Mohan, V;Opal, P;
    Journal of neuroinflammation
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Chronic ethanol induces a pro-inflammatory switch in interleukin-1beta regulation of GABAergic signaling in the medial prefrontal cortex of male mice
    Authors: FP Varodayan, AR Pahng, TD Davis, P Gandhi, M Bajo, MQ Steinman, WB Kiosses, YA Blednov, MD Burkart, S Edwards, AJ Roberts, M Roberto
    Brain, Behavior, and Immunity, 2023-02-28;110(0):125-139.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation
    Authors: A Villatoro, V Cuminetti, A Bernal, C Torroja, I Cossío, A Benguría, M Ferré, J Konieczny, E Vázquez, A Rubio, P Utnes, X You, CG Fenton, RH Paulssen, J Zhang, F Sánchez-Ca, A Dopazo, A Vik, E Anderssen, A Hidalgo, L Arranz
    Nature Communications, 2023-01-03;14(1):12.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Chronic ethanol induces a pro-inflammatory switch in interleukin-1beta regulation of GABAergic signaling in the medial prefrontal cortex of male mice
    Authors: FP Varodayan, AR Pahng, TD Davis, P Gandhi, M Bajo, MQ Steinman, WB Kiosses, YA Blednov, MD Burkart, S Edwards, AJ Roberts, M Roberto
    Brain, Behavior, and Immunity, 2023;110(0):125-139.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Interleukin-1 contributes to clonal expansion and progression of bone marrow fibrosis in JAK2V617F-induced myeloproliferative neoplasm
    Authors: MF Rahman, Y Yang, BT Le, A Dutta, J Posyniak, P Faughnan, MA Sayem, NS Aguilera, G Mohi
    Nature Communications, 2022-09-13;13(1):5347.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia
    Authors: R Furrer, AJ Jauch, T Nageswara, S Dilbaz, P Rhein, SA Steurer, M Recher, RC Skoda, C Handschin
    Science Advances, 2021-09-08;7(37):eabi4852.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  8. Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
    Authors: A Shimada, S Hasegawa-I
    Toxicology reports, 2021-03-10;8(0):520-528.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?
    Authors: HM Hsiao, R Fernandez, S Tanaka, W Li, JH Spahn, S Chiu, M Akbarpour, D Ruiz-Perez, Q Wu, C Turam, D Scozzi, T Takahashi, HP Luehmann, V Puri, GRS Budinger, AS Krupnick, AV Misharin, KJ Lavine, Y Liu, AE Gelman, A Bharat, D Kreisel
    J. Clin. Invest., 2018-05-21;128(7):2833-2847.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Spinal astrocytic activation contributes to mechanical allodynia in a rat model of cyclophosphamide-induced cystitis
    Mol Pain, 2016-11-15;12(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Neutrophils mediate blood-spinal cord barrier disruption in demyelinating neuroinflammatory diseases.
    Authors: Aube B, Levesque S, Pare A, Chamma E, Kebir H, Gorina R, Lecuyer M, Alvarez J, De Koninck Y, Engelhardt B, Prat A, Cote D, Lacroix S
    J Immunol, 2014-07-21;193(5):2438-54.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice.
    Authors: Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M
    PLoS ONE, 2013-11-26;8(11):e81744.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012-05-21;209(6):1219-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Interleukin-1beta causes long-term potentiation deficiency in a mouse model of septic encephalopathy.
    Authors: Imamura Y, Wang H, Matsumoto N, Muroya T, Shimazaki J, Ogura H, Shimazu T
    Neuroscience, 2011-05-07;187(0):63.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  16. Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet.
    Authors: Grayson BE, Levasseur PR, Williams SM, Smith MS, Marks DL, Grove KL
    Endocrinology, 2010-02-22;151(4):1622-32.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
    Authors: O&amp;apos;Connor JC, Sherry CL, Guest CB, Freund GG
    J. Immunol., 2007-06-01;178(11):6886-93.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: ELISA Development
  18. IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons.
    Authors: Davis CN, Tabarean I, Gaidarova S, Behrens MM, Bartfai T
    J. Neurochem., 2006-06-12;98(5):1379-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse IL-1 RI Antibody

Average Rating: 2.3 (Based on 3 Reviews)

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Mouse IL-1 RI Antibody
By Anonymous on 09/09/2019
Application: WB Sample Tested: mouse pancreatic cell line Species: Mouse

No band at all. (cell lysates: loaded 35ug protein, PicoPlus substrate 5min, exposed for 20min)
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***


Mouse IL-1 RI Antibody
By Anonymous on 08/09/2018
Application: ELISA Sample Tested: Brain (hippocampus) tissue Species: Mouse

Mouse IL-1 RI Antibody
By Anonymous on 08/07/2018
Sample Tested: Brain (hippocampus) tissue Species: Mouse

Would not stain, had to switch to a different antibody.
RnD Systems Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.