Mouse IL-2 Antibody

Catalog # Availability Size / Price Qty
AB-402-NA
Mouse IL-2 Antibody in Data
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Citations (1)
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Mouse IL-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human IL-2, recombinant rat IL-2, recombinant equine IL-2, recombinant porcine IL-2 and recombinant bovine IL-2 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse IL-2
Ala21-Gln169
Accession # P04351
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑2 (Catalog # 402-ML)
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line [Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276]. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 2 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑2 Polyclonal Antibody (Catalog # AB-402-NA). The ND50is typically 2-8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1‑3). Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch (4). Mouse and human IL-2 exhibit cross-species activity (5). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (6‑8). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (9). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (10‑12). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Kashima, N. et al. (1985) Nature 313:402.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  11. Laurence, A. et al. (2007) Immunity 26:371.
  12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Citation for Mouse IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
    Authors: Bednarski JJ, Warner RE, Rao T
    Arthritis Rheum., 2003;48(3):757-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development

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