Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse KLF4 Ala11-Phe483 Accession # Q60793
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Mouse KLF4
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.
KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
KLF4 belongs to the KLF family of transcription factors that regulate
several cell functions. It is highly expressed in differentiated
epithelial cells of the gastrointestinal tract. Human and mouse KLF4
share 91% amino acid sequence homology.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
MEFs expressing indicated constructs were lysed and total lysates run on 10% gels. Blots were blocked in 5% milk in TBST, incubated with antibody in 1% milk in TBST over night at 4C, washed 3x in TBST for 15min, incubated with HRP-conjugated secondary antibody in 1% milk in TBST for 1h, washed again 3x in TBST for 15min and then developed. Bands in other than Klf4 lane show endogenous Klf4 proteins. Specificity: Specific Sensitivity: Sensitive Buffer: TBST + 1% milk Dilution: 1:1000
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.