Mouse KLF4 Antibody

(20 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse KLF4
    Ala11-Phe483
    Accession # Q60793
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse KLF4
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chromatin Immunoprecipitation (ChIP)
Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.
Immunocytochemistry
KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF4
KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.
  • Long Name:
    Kruppel-Like Factor 4
  • Entrez Gene IDs:
    9314 (Human)
  • Alternate Names:
    Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017;2017(0):4745252.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
    Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
    Nat Commun, 2017;8(0):15397.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs
    Authors: S Gao, X Hou, Y Jiang, Z Xu, T Cai, J Chen, G Chang
    Stem Cell Res Ther, 2017;8(1):13.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.
    Authors: Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis S
    PLoS ONE, 2015;10(5):e0126475.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IP
  7. Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development.
    Authors: Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D
    PLoS ONE, 2015;10(7):e0134390.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  8. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014;3(1):156-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014;32(5):1149-60.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.
    Authors: Hale A, Tian H, Anih E, Recio F, Shatat M, Johnson T, Liao X, Ramirez-Bergeron D, Proweller A, Ishikawa M, Hamik A
    J Biol Chem, 2014;289(17):12016-28.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Citrullination regulates pluripotency and histone H1 binding to chromatin.
    Authors: Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T
    Nature, 2014;507(7490):104-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  12. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  13. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014;322(2):335-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  14. Kruppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation.
    Authors: Karpurapu M, Ranjan R, Deng J, Chung S, Lee Y, Xiao L, Nirujogi T, Jacobson J, Park G, Christman J
    PLoS ONE, 2014;9(4):e93362.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  15. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013;12(6):699-712.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
  16. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013;126(0):1093-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  17. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
    Authors: Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P
    PLoS ONE, 2012;7(4):e35892.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  18. Sirt1, p53, and p38(MAPK) Are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation.
    Authors: Hishida T, Nozaki Y, Nakachi Y, Mizuno Y, Iseki H, Katano M, Kamon M, Hirasaki M, Nishimoto M, Okazaki Y, Okuda A
    Stem Cells, 2012;30(8):1634-44.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. E-cadherin and, in its absence, N-cadherin promotes Nanog expression in mouse embryonic stem cells via STAT3 phosphorylation.
    Authors: Hawkins K, Mohamet L, Ritson S, Merry C, Ward C
    Stem Cells, 2012;30(9):1842-51.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state.
    Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F
    Stem Cells, 2010;28(2):191-200.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 20 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 2 reviews)

Have you used Mouse KLF4 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse KLF4 Antibody.

We have 1 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: Western Blot.

Filter by Ratings
Excellent
(2)
All Ratings
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Anonymous 02/22/2016
Show

Summary

ApplicationWestern Blot
Sample TestedMouse intestinal tissue,Human colorectal cancer tissue,HCT116,Mouse embryonic fibroblasts,Caco-2 human colorectal adenocarcinoma cell line
Western Blot Mouse KLF4 Antibody AF3158
Enlarge
Excellent
 WB Mouse Anonymous 10/26/2015
Show
Image Details
Western Blot Mouse KLF4 Antibody AF3158
Western Blot: Mouse KLF4 Antibody [AF3158]

Summary

ApplicationWestern Blot
Sample TestedMouse embryonic fibroblasts
SpeciesMouse

Other Experimental Details

Other Experimental DetailsMEFs expressing indicated constructs were lysed and total lysates run on 10% gels.
Blots were blocked in 5% milk in TBST, incubated with antibody in 1% milk in TBST over night at 4C, washed 3x in TBST for 15min, incubated with HRP-conjugated secondary antibody in 1% milk in TBST for 1h, washed again 3x in TBST for 15min and then developed.
Bands in other than Klf4 lane show endogenous Klf4 proteins.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBST + 1% milk
Dilution: 1:1000

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information