Mouse KLF4 Antibody

Catalog # Availability Size / Price Qty
AF3158
AF3158-SP
Mouse KLF4 Antibody in Chromatin Immunoprecipitation (ChIP)
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Product Details
Citations (32)
FAQs
Supplemental Products
Reviews (2)

Mouse KLF4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 20% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse KLF4
Ala11-Phe483
Accession # Q60793
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse KLF4
Immunohistochemistry
1-25 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Chromatin Immunoprecipitation (ChIP) Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheB2Rpromoter was detected by standard PCR.

Immunocytochemistry KLF4 in D3 Mouse Cell Line. View Larger

KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry KLF4 in Mouse Colon Tissue. View Larger

KLF4 in Mouse Colon Tissue. KLF4 was detected in perfusion fixed frozen sections of mouse colon tissue using Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF4

KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

Long Name
Kruppel-Like Factor 4
Entrez Gene IDs
9314 (Human)
Alternate Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut)

Product Datasheets

Citations for Mouse KLF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency
    Authors: T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith
    Cell Stem Cell, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Kr�ppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation
    Authors: CC Estrada, P Paladugu, Y Guo, J Pace, MP Revelo, DJ Salant, SJ Shankland, VD D'Agati, A Mehrotra, S Cardona, AB Bialkowska, VW Yang, JC He, SK Mallipattu
    JCI Insight, 2018;3(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer
    Authors: X Xiong, M Schober, E Tassone, A Khodadadi-, A Sastre-Per, H Zhou, A Tsirigos, S Shen, M Chang, J Melamed, L Ossowski, EL Wilson
    Cell Rep, 2018;25(11):3006-3020.e7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate
    Authors: JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf
    Cell Stem Cell, 2018;0(0):.
  6. Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells
    Authors: R Fajka-Boja, A Marton, A Tóth, P Blazsó, V Tubak, B Bálint, I Nagy, Z Heged?s, C Vizler, RL Katona
    BMC Cancer, 2018;18(1):872.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. GLK-IKK? signaling induces dimerization and translocation of the AhR-ROR?t complex in IL-17A induction and autoimmune disease
    Authors: HC Chuang, CY Tsai, CH Hsueh, TH Tan
    Sci Adv, 2018;4(9):eaat5401.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  8. RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation
    Authors: F Bustos, A Segarra-Fa, VK Chaugule, L Brandenbur, E Branigan, R Toth, T Macartney, A Knebel, RT Hay, H Walden, GM Findlay
    Cell Rep, 2018;23(6):1599-1611.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency
    Authors: K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler
    Cell Stem Cell, 2018;0(0):.
  10. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function
    Authors: C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta
    Data Brief, 2018;16(0):929-938.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension
    Authors: AQ Sheikh, FZ Saddouk, A Ntokou, R Mazurek, DM Greif
    Cell Rep, 2018;23(4):1152-1165.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
    Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
    Nat Commun, 2017;8(0):15397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs
    Authors: S Gao, X Hou, Y Jiang, Z Xu, T Cai, J Chen, G Chang
    Stem Cell Res Ther, 2017;8(1):13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017;2017(0):4745252.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
    Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
    Cell Death Discov, 2017;3(0):16105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.
    Authors: Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis S
    PLoS ONE, 2015;10(5):e0126475.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  19. Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development.
    Authors: Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D
    PLoS ONE, 2015;10(7):e0134390.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  21. Kruppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation.
    Authors: Karpurapu M, Ranjan R, Deng J, Chung S, Lee Y, Xiao L, Nirujogi T, Jacobson J, Park G, Christman J
    PLoS ONE, 2014;9(4):e93362.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Citrullination regulates pluripotency and histone H1 binding to chromatin.
    Authors: Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T
    Nature, 2014;507(7490):104-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  23. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.
    Authors: Hale A, Tian H, Anih E, Recio F, Shatat M, Johnson T, Liao X, Ramirez-Bergeron D, Proweller A, Ishikawa M, Hamik A
    J Biol Chem, 2014;289(17):12016-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014;32(5):1149-60.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014;322(2):335-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  26. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014;3(1):156-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013;12(6):699-712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  28. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013;126(0):1093-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
    Authors: Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P
    PLoS ONE, 2012;7(4):e35892.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  30. E-cadherin and, in its absence, N-cadherin promotes Nanog expression in mouse embryonic stem cells via STAT3 phosphorylation.
    Authors: Hawkins K, Mohamet L, Ritson S, Merry C, Ward C
    Stem Cells, 2012;30(9):1842-51.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Sirt1, p53, and p38(MAPK) Are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation.
    Authors: Hishida T, Nozaki Y, Nakachi Y, Mizuno Y, Iseki H, Katano M, Kamon M, Hirasaki M, Nishimoto M, Okazaki Y, Okuda A
    Stem Cells, 2012;30(8):1634-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state.
    Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F
    Stem Cells, 2010;28(2):191-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Mouse KLF4 Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Mouse intestinal tissue,Human colorectal cancer tissue,HCT116,Mouse embryonic fibroblasts,Caco-2 human colorectal adenocarcinoma cell line Species: Mouse,Human

Mouse KLF4 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse embryonic fibroblasts Species: Mouse

MEFs expressing indicated constructs were lysed and total lysates run on 10% gels.
Blots were blocked in 5% milk in TBST, incubated with antibody in 1% milk in TBST over night at 4C, washed 3x in TBST for 15min, incubated with HRP-conjugated secondary antibody in 1% milk in TBST for 1h, washed again 3x in TBST for 15min and then developed.
Bands in other than Klf4 lane show endogenous Klf4 proteins.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBST + 1% milk
Dilution: 1:1000