Mouse KLF4 Antibody

Catalog # Availability Size / Price Qty
AF3158
AF3158-SP
Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation.
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Product Details
Citations (47)
FAQs
Supplemental Products
Reviews (2)

Mouse KLF4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse KLF4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse KLF4
Ala11-Phe483
Accession # Q60793
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse KLF4
Immunohistochemistry
1-25 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Chromatin Immunoprecipitation (ChIP) Detection of KLF4-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheB2Rpromoter was detected by standard PCR.

Immunocytochemistry KLF4 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry KLF4 antibody in Mouse Colon Tissue by Immunohistochemistry (IHC-Fr). View Larger

KLF4 in Mouse Colon Tissue. KLF4 was detected in perfusion fixed frozen sections of mouse colon tissue using Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Detection of Mouse KLF4 by Immunohistochemistry View Larger

Detection of Mouse KLF4 by Immunohistochemistry Overexpression of beta -catenin maintains B6 ESC self-renewal in the presence of LIF.(A) Immunoblot analysis of phospho-beta -catenin, beta -catenin and beta -actin levels in B6 ES cells cultured in 1000 U/ml LIF condition supplemented without or with 3 µM CH for 4 days. (B) Immunofluorescence analysis of 3 µM CH-treated and non-treated B6 ESC clones stained for beta -catenin and Hoechst for nuclei in the presence of LIF for 4 days. Scale bars represent 100 µm. (C) Quantitative RT-PCR analysis of Axin2, T and Cdx1 transcript levels in 3 µM CH-treated and control B6 ES cells. Error bars represent the SD of three biological replicates. (D) beta -catenin S33A protein stably expressed in ES cells was detected by immunoblotting. (E) Phase contrast microscopy image and alkaline phosphatase staining of control and beta -catenin S33A-transfected B6 ES cells in the presence of 1000 U/ml LIF for 4 days. Scale bars represent 100 µm. (F) Immunofluorescence staining for OCT4, NANOG, and KLF4 in mock and beta -catenin S33A-transfected B6 ES cells in the presence of 1000 U/ml LIF for 4 days. Cell nuclei were stained with Hoechst. Scale bars represent 100 µm. (G) Quantitative RT-PCR analysis of Oct4, Nanog, Rex1, Klf4, Tbx3, and Fgf5 transcript levels in mock and beta -catenin S33A-transfected B6 ES cells in the presence of 1000 U/ml LIF for 4 days. Error bars represent the SD of three biological replicates. (H) beta -catenin S33A delta C, beta -catenin S33A and TCF4 delta N protein were detected by immunoblotting with anti-flag tag antibody from extracts of ES cells transfected with PiggyBac plasmids. Quantitative RT-PCR analysis of Axin2, T and Cdx1 transcript levels in beta -catenin S33A delta C, beta -catenin S33A, TCF4 delta N, and mock-transfected B6 ES cells. Error bars represent the SD of three biological replicates. (I) Alkaline phosphatase staining of mock-transfected, and beta -catenin delta C over-expressing B6 ES cells cultured in LIF for 5 passages, and mock-transfected and TCF4 delta N-transfected B6 ESCs cultured in CHIR99021 plus LIF for 5 passages. Scale bars represent 100 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0035892), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse KLF4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse KLF4 by Immunocytochemistry/Immunofluorescence LIF maintains B6 ES cell self-renewal in the presence of CHIR99021.(A) Phase contrast image of B6 ES cells in the presence of 1000 U/ml LIF supplemented without or with 3 µM CHIR99021 (CH) for 4 days. Scale bars represent 100 µm. (B) Alkaline phosphatase staining of B6 ES cells in the presence of 1000 U/ml LIF supplemented without or with 3 µM CH for 4 days. Scale bars represent 100 µm. (C) Immunofluorescence staining for OCT4, NANOG, and KLF4 in B6 ES cells treated with 1000 U/ml LIF and 3 µM CH for 4 days. Cell nuclei were stained using Hoechst. Scale bars represent 100 µm. (D) Relative quantification of Oct4, Nanog, Rex1, Klf4, Tbx3, and Fgf5 mRNA in B6 ES cells in 1000 U/ml LIF culture without or with 3 µM CH, by qRT-PCR. Error bars represent the SD of three biological replicates. (E) B6 ES cells,1×104/well, cultured in different concentrations of LIF, ranging from 0 to 10,000 U/ml, in the absence or presence of 3 µM CH for 5 days, were assessed for alkaline phosphatase activity. Scale bars represent 100 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0035892), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chromatin Immunoprecipitation Detection of Mouse KLF4 by Chromatin Immunoprecipitation View Larger

Detection of Mouse KLF4 by Chromatin Immunoprecipitation Overlapping SIM2 occupancy with master transcription factor binding sites.a. Frequency distribution of OCT4, SOX2 and NANOG DNA binding sites in a 40kb window centered to the newly identified SIM2 DNA binding sites. Plots show a significant enrichment for the OSN binding sites at the SIM2 peak localization in SIM2 A6 expressing cells. Pie charts show the proportion of SIM2 DNA binding sites overlapping with the OCT4, SOX2 or NANOG binding sites (in grey) (100bp window). p = Fisher’s exact test p-value; F score: measure of the significance of the association (1 = perfect match). b. Protein co-immunoprecipitation experiments of SIM2-FLAG with endogenous OCT4, SOX2, KLF4 (left panel) and NANOG (right panel). Cellular protein extracts from Sim2 expressing cells (A6) or EB3 cells were immunoprecipitated by using antibodies directed against each of the pluripotency factors (N-terminal and C-terminal part of NANOG) or IgG as a negative control for co-immunoprecipitation. Associated proteins were immunoblotted using an anti-FLAG antibody. Red star shows the SIM2-FLAG protein, blue star the signal given by the recognition of the IgG heavy chains. Ø: Beads only; kDa: kilodaltons; protein lysat: protein lysat was loaded as an input control for the immunoblot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25955728), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF4

KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

Long Name
Kruppel-Like Factor 4
Entrez Gene IDs
9314 (Human)
Alternate Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut)

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Citations for Mouse KLF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation
    Authors: X Yang, ST Wu, R Gao, R Wang, Y Wang, Z Dong, L Wang, C Qi, X Wang, ML Schmitz, R Liu, Z Han, L Wang, X Zheng
    JCI Insight, 2023-03-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomes
    Authors: S Brahma, S Henikoff
    bioRxiv : the preprint server for biology, 2023-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. KLF4 inhibits early neural differentiation of ESCs by coordinating specific 3D chromatin structure
    Authors: J Bi, W Wang, M Zhang, B Zhang, M Liu, G Su, F Chen, B Chen, T Shi, Y Zheng, X Zhao, Z Zhao, J Shi, P Li, L Zhang, W Lu
    Nucleic Acids Research, 2022-11-28;50(21):12235-12250.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. BMP4 drives primed to na�ve transition through PGC-like state
    Authors: S Yu, C Zhou, J He, Z Yao, X Huang, B Rong, H Zhu, S Wang, S Chen, X Wang, B Cai, G Zhao, Y Chen, L Xiao, H Liu, Y Qin, J Guo, H Wu, Z Zhang, M Zhang, X Zhao, F Lan, Y Wang, J Chen, S Cao, D Pei, J Liu
    Nature Communications, 2022-05-19;13(1):2756.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Multiplexed genome regulation in vivo with hyper-efficient Cas12a
    Authors: LY Guo, J Bian, AE Davis, P Liu, HR Kempton, X Zhang, A Chemparath, B Gu, X Lin, DA Rane, X Xu, RM Jamiolkows, Y Hu, S Wang, LS Qi
    Nature Cell Biology, 2022-04-12;24(4):590-600.
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: ICC
  6. Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm
    Authors: ZY Zuo, GH Yang, HY Wang, SY Liu, YJ Zhang, Y Cai, F Chen, H Dai, Y Xiao, MB Cheng, Y Huang, Y Zhang
    Nucleic Acids Research, 2022-02-28;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs
    Authors: J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle
    Nature Communications, 2021-12-16;12(1):7322.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  8. Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization
    Authors: S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas
    Cell Stem Cell, 2021-06-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Principles of signaling pathway modulation for enhancing human naive pluripotency induction
    Authors: J Bayerl, M Ayyash, T Shani, YS Manor, O Gafni, R Massarwa, Y Kalma, A Aguilera-C, M Zerbib, H Amir, D Sheban, S Geula, N Mor, L Weinberger, S Naveh Tass, V Krupalnik, B Oldak, N Livnat, S Tarazi, S Tawil, E Wildschutz, S Ashouokhi, L Lasman, V Rotter, S Hanna, D Ben-Yosef, N Novershter, S Viukov, JH Hanna
    Cell Stem Cell, 2021-04-28;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP, Western Blot
  10. Loss of mucosal p32/gC1qR/HABP1 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitis
    Authors: A Sünderhauf, M Hicken, H Schlichtin, K Skibbe, M Ragab, A Raschdorf, M Hirose, H Schäffler, A Bokemeyer, D Bettenwort, AG Savitt, S Perner, S Ibrahim, EL Peerschke, B Ghebrehiwe, S Derer, C Sina
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  11. Reprogramming to recover youthful epigenetic information and restore vision
    Authors: Y Lu, B Brommer, X Tian, A Krishnan, M Meer, C Wang, DL Vera, Q Zeng, D Yu, MS Bonkowski, JH Yang, S Zhou, EM Hoffmann, MM Karg, MB Schultz, AE Kane, N Davidsohn, E Korobkina, K Chwalek, LA Rajman, GM Church, K Hochedling, VN Gladyshev, S Horvath, ME Levine, MS Gregory-Ks, BR Ksander, Z He, DA Sinclair
    Nature, 2020-12-02;588(7836):124-129.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Interplay among p21Waf1/Cip1, MUSASHI-1 and Kr�ppel-like factor 4 in activation of Bmi1-CreER reserve intestinal stem cells after gamma radiation-induced injury
    Authors: EJ Orzechowsk, T Katano, AB Bialkowska, VW Yang
    Sci Rep, 2020-10-27;10(1):18300.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging
    Authors: Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog
    Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  14. Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy
    Authors: B He, A Johansson-, J Backhouse, J Li, GYF Lee, J Hamzah, R Ganss
    Cell Rep, 2020-01-21;30(3):714-724.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019-06-27;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Endothelial cell clonal expansion in the development of cerebral cavernous malformations
    Authors: M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana
    Nat Commun, 2019-06-24;10(1):2761.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency
    Authors: T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith
    Cell Stem Cell, 2019-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer
    Authors: X Xiong, M Schober, E Tassone, A Khodadadi-, A Sastre-Per, H Zhou, A Tsirigos, S Shen, M Chang, J Melamed, L Ossowski, EL Wilson
    Cell Rep, 2018-12-11;25(11):3006-3020.e7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  19. Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate
    Authors: JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf
    Cell Stem Cell, 2018-09-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  20. GLK-IKK? signaling induces dimerization and translocation of the AhR-ROR?t complex in IL-17A induction and autoimmune disease
    Authors: HC Chuang, CY Tsai, CH Hsueh, TH Tan
    Sci Adv, 2018-09-12;4(9):eaat5401.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  21. Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells
    Authors: R Fajka-Boja, A Marton, A Tóth, P Blazsó, V Tubak, B Bálint, I Nagy, Z Heged?s, C Vizler, RL Katona
    BMC Cancer, 2018-09-05;18(1):872.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Kr�ppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation
    Authors: CC Estrada, P Paladugu, Y Guo, J Pace, MP Revelo, DJ Salant, SJ Shankland, VD D'Agati, A Mehrotra, S Cardona, AB Bialkowska, VW Yang, JC He, SK Mallipattu
    JCI Insight, 2018-06-21;3(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency
    Authors: K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler
    Cell Stem Cell, 2018-06-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  24. RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation
    Authors: F Bustos, A Segarra-Fa, VK Chaugule, L Brandenbur, E Branigan, R Toth, T Macartney, A Knebel, RT Hay, H Walden, GM Findlay
    Cell Rep, 2018-05-08;23(6):1599-1611.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension
    Authors: AQ Sheikh, FZ Saddouk, A Ntokou, R Mazurek, DM Greif
    Cell Rep, 2018-04-24;23(4):1152-1165.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function
    Authors: C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta
    Data Brief, 2017-12-13;16(0):929-938.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017-09-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017-07-05;2017(0):4745252.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
    Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
    Nat Commun, 2017-06-07;8(0):15397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  31. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs
    Authors: S Gao, X Hou, Y Jiang, Z Xu, T Cai, J Chen, G Chang
    Stem Cell Res Ther, 2017-01-23;8(1):13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development.
    Authors: Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D
    PLoS ONE, 2015-07-30;10(7):e0134390.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells.
    Authors: Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis S
    PLoS ONE, 2015-05-08;10(5):e0126475.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  34. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014-07-25;124(9):3960-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014-06-06;3(1):156-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  36. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014-05-01;32(5):1149-60.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Kruppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation.
    Authors: Karpurapu M, Ranjan R, Deng J, Chung S, Lee Y, Xiao L, Nirujogi T, Jacobson J, Park G, Christman J
    PLoS ONE, 2014-04-02;9(4):e93362.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.
    Authors: Hale A, Tian H, Anih E, Recio F, Shatat M, Johnson T, Liao X, Ramirez-Bergeron D, Proweller A, Ishikawa M, Hamik A
    J Biol Chem, 2014-03-05;289(17):12016-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014-02-18;322(2):335-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  40. Bidirectional developmental potential in reprogrammed cells with acquired pluripotency.
    Authors: Obokata, Haruko, Sasai, Yoshiki, Niwa, Hitoshi, Kadota, Mitsutak, Andrabi, Munazah, Takata, Nozomu, Tokoro, Mikiko, Terashita, Yukari, Yonemura, Shigenob, Vacanti, Charles, Wakayama, Teruhiko
    Nature, 2014-01-30;505(7485):676-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  41. Citrullination regulates pluripotency and histone H1 binding to chromatin.
    Authors: Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T
    Nature, 2014-01-26;507(7490):104-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  42. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013-05-09;12(6):699-712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP, Co-Immunoprecipitation
  43. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013-01-23;126(0):1093-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  44. E-cadherin and, in its absence, N-cadherin promotes Nanog expression in mouse embryonic stem cells via STAT3 phosphorylation.
    Authors: Hawkins K, Mohamet L, Ritson S, Merry C, Ward C
    Stem Cells, 2012-09-01;30(9):1842-51.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  45. Sirt1, p53, and p38(MAPK) Are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation.
    Authors: Hishida T, Nozaki Y, Nakachi Y, Mizuno Y, Iseki H, Katano M, Kamon M, Hirasaki M, Nishimoto M, Okazaki Y, Okuda A
    Stem Cells, 2012-08-01;30(8):1634-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  46. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
    Authors: Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P
    PLoS ONE, 2012-04-23;7(4):e35892.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  47. Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state.
    Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F
    Stem Cells, 2010-02-01;28(2):191-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Mouse KLF4 Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Caco-2 human colorectal adenocarcinoma cell line,HCT116,Human colorectal cancer tissue,Mouse embryonic fibroblasts,Mouse intestinal tissue Species: Human and Mouse

Mouse KLF4 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse embryonic fibroblasts Species: Mouse

MEFs expressing indicated constructs were lysed and total lysates run on 10% gels.
Blots were blocked in 5% milk in TBST, incubated with antibody in 1% milk in TBST over night at 4C, washed 3x in TBST for 15min, incubated with HRP-conjugated secondary antibody in 1% milk in TBST for 1h, washed again 3x in TBST for 15min and then developed.
Bands in other than Klf4 lane show endogenous Klf4 proteins.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBST + 1% milk
Dilution: 1:1000