Mouse NGF R/TNFRSF16 Antibody

Catalog # Availability Size / Price Qty
AF1157
AF1157-SP
Detection of  Mouse NGF R/TNFRSF16 by Western Blot.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (4)

Mouse NGF R/TNFRSF16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NGF R/TNFRSF16 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NGF R/TNFRSF16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NGF R/TNFRSF16
Gly20-Asn243
Accession # Q9Z0W1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of  Mouse NGF R/TNFRSF16 by Western Blot. View Larger

Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately
60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry NGF R/TNFRSF16 in Mouse Brain. View Larger

NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGF R/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3).

References
  1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
  2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
  3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Long Name
Nerve Growth Factor Receptor
Entrez Gene IDs
4804 (Human); 18053 (Mouse); 117089 (Rat)
Alternate Names
CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16

Product Datasheets

Citations for Mouse NGF R/TNFRSF16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
    Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
    Sci Rep, 2017;7(1):5127.
    Species: Human
    Sample Types: Uring
    Applications: ELISA detection
  2. Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide
    Authors: K Szklany, E Ruiter, F Mian, W Kunze, J Bienenstoc, P Forsythe, K Karimi
    PLoS ONE, 2016;11(3):e0152443.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  3. An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
    Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
    J Biol Chem, 2013;288(16):11144-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
    Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
    PLoS ONE, 2010;5(12):e15053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  5. Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
    Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
    Mol. Ther., 2010;18(12):2164-72.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC Frozen

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Reviews for Mouse NGF R/TNFRSF16 Antibody

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Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 11/02/2017
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse

Postnatal day 7 mouse cerebellar tissue.


Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 10/17/2016
Application: IHC Sample Tested: Brain (cortex) tissue Species: Mouse

PN18 mouseClosed Head InjuryDilution 1:500


Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 07/26/2016
Application: IHC Sample Tested: Brain (cerebral cortex) Species: Mouse

Frozen section. 20um thickness. Antibody dilution 1/500.


Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 07/13/2016
Application: IHC Sample Tested: Cerebellum tissue Species: Rat,Mouse

Cryostat Frozen section. 20um ticknes. Antibody dilution 1/500 ON 4C.