Mouse NGF R/TNFRSF16 Antibody

(5 citations)
(4 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse NGF R/TNFRSF16 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NGF R/TNFRSF16 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse NGF R/TNFRSF16
    Gly20-Asn243
    Accession # Q9Z0W1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately
60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NGF R/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3).

  • References:
    1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
    2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
    3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
  • Long Name:
    Nerve Growth Factor Receptor
  • Entrez Gene IDs:
    4804 (Human); 18053 (Mouse); 117089 (Rat)
  • Alternate Names:
    CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
    Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
    Sci Rep, 2017;7(1):5127.
    Species: Human
    Sample Type: Uring
    Application: ELISA detection
  2. Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide
    Authors: K Szklany, E Ruiter, F Mian, W Kunze, J Bienenstoc, P Forsythe, K Karimi
    PLoS ONE, 2016;11(3):e0152443.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
    Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
    J Biol Chem, 2013;288(16):11144-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
    Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
    Mol. Ther., 2010;18(12):2164-72.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
    Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
    PLoS ONE, 2010;5(12):e15053.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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Average Rating: 5 (Based on 4 reviews)

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We have 3 reviews tested in 1 species: Mouse.
We have 4 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
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Excellent
 IHC Mouse Anonymous 11/02/2017
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Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Immunohistochemistry: Mouse NGF R/TNFRSF16 Antibody [AF1157]

Summary

ApplicationImmunohistochemistry
Sample TestedCerebellum tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsPostnatal day 7 mouse cerebellar tissue.
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
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Excellent
 IHC Mouse Anonymous 10/17/2016
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Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Immunohistochemistry: Mouse NGF R/TNFRSF16 Antibody [AF1157]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain (cortex) tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsPN18 mouseClosed Head InjuryDilution 1:500
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
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Excellent
 IHC Mouse Anonymous 07/26/2016
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Image Details
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Immunohistochemistry: Mouse NGF R/TNFRSF16 Antibody [AF1157]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain (cerebral cortex)
SpeciesMouse

Other Experimental Details

Other Experimental DetailsFrozen section. 20um thickness. Antibody dilution 1/500.
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
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Excellent
 IHC Anonymous 07/13/2016
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Image Details
Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Immunohistochemistry: Mouse NGF R/TNFRSF16 Antibody [AF1157]

Summary

ApplicationImmunohistochemistry
Sample TestedCerebellum tissue

Other Experimental Details

Other Experimental DetailsCryostat Frozen section. 20um ticknes. Antibody dilution 1/500 ON 4C.

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