Mouse PD-L1/B7-H1 Antibody

Catalog # Availability Size / Price Qty
AF1019
AF1019-SP
Detection of Mouse PD-L1/B7-H1 by Western Blot.
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Product Details
Citations (30)
FAQs
Supplemental Products
Reviews (10)

Mouse PD-L1/B7-H1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
Phe19-Thr238
Accession # Q9EP73
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PD-L1/B7-H1 antibody by Western Blot. View Larger

Detection of Mouse PD-L1/B7-H1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PD-L1/B7-H1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PD-L1/B7-H1 antibody in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

Western Blot Detection of Mouse PD-L1 by Western Blot View Larger

Detection of Mouse PD-L1 by Western Blot Pyruvate kinase isoform M2 (PKM2) is required for LPS-induced expression of PD-L1. RAW cells (0.5 × 106 cells/ml) (A), bone marrow-derived macrophages (BMDMs) (B), or bone marrow-derived dendritic cells (C) were treated with TEPP-46 at 50 µM for 1 h prior to stimulation with LPS (100 ng/ml, 24 h), lysed and assayed for expression of PD-L1 by flow cytometry [(A–C) top panels] and pdl1 mRNA by RT-PCR [(A,B) bottom panels]. Statistical analysis was performed on cells from three separate experiments. Error bars represent mean ± SEM. BMDM cells were transfected with scrambled control (SC) or anti-PKM2 siRNA (D). 24 h post-transfection, cells were stimulated with LPS (100 ng/ml) for 24 h after which expression of PKM2 (top panel) and PDL1 (middle panel) was measured by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29081778), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1‑4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7‑H1/PD‑L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1‑4).

References
  1. Tamura, H. et al. (2001) Blood 97:1809.
  2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
  3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

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Citations for Mouse PD-L1/B7-H1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Design, synthesis, and biological activity of dual monoamine oxidase A and heat shock protein 90 inhibitors, N-Methylpropargylamine-conjugated 4-isopropylresorcinol for glioblastoma
    Authors: Tseng, HJ;Banerjee, S;Qian, B;Lai, MJ;Wu, TY;Hsu, TI;Lin, TE;Hsu, KC;Chuang, KH;Liou, JP;Shih, JC;
    European journal of medicinal chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer
    Authors: Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E;
    Oncogenesis
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Angiogenic inhibitor pre-administration improves the therapeutic effects of immunotherapy
    Authors: M Sato, N Maishi, Y Hida, A Yanagawa-M, MT Alam, J Sakakibara, JM Nam, Y Onodera, S Konno, K Hida
    Cancer Medicine, 2023-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNA
    Authors: Zhang, W;Pan, X;Xu, Y;Guo, H;Zheng, M;Chen, X;Wu, H;Luan, F;He, Q;Ding, L;Yang, B;
    Acta Pharmaceutica Sinica B
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Pancreatic Islet Cells Response to IFNgamma Relies on Their Spatial Location within an Islet
    Authors: M De Burghgr, C Lourenço, C Berthault, V Aiello, A Villalba, A Fouque, M Diedisheim, S You, M Oshima, R Scharfmann
    Cells, 2022-12-28;12(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity
    Authors: TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi
    JCI Insight, 2022-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Western Blot
  7. A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells
    Authors: Y Li, L Wu, Y Liu, S Ma, B Huang, X Feng, H Wang
    Translational Oncology, 2022-04-26;21(0):101424.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA
  8. Mitogen-Activated Protein Kinase Phosphatase-1 Controls PD-L1 Expression by Regulating Type I Interferon during Systemic Escherichia coli Infection
    Authors: TJ Barley, PR Murphy, X Wang, BA Bowman, JM Mormol, CE Mager, SG Kirk, CJ Cash, SC Linn, X Meng, LD Nelin, B Chen, M Hafner, J Zhang, Y Liu
    The Journal of Biological Chemistry, 2022-04-13;0(0):101938.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. PD-L1 near Infrared Photoimmunotherapy of Ovarian Cancer Model
    Authors: J Jin, I Sivakumar, Y Mironchik, B Krishnamac, F Wildes, JD Barnett, CF Hung, S Nimmagadda, H Kobayashi, ZM Bhujwalla, MF Penet
    Cancers, 2022-01-26;14(3):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Anti?VEGF antibody triggers the effect of anti?PD?L1 antibody in PD?L1low and immune desert?like mouse tumors
    Authors: N Ishikura, M Sugimoto, K Yorozu, M Kurasawa, O Kondoh
    Oncology reports, 2021-12-27;47(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. A Novel Kinase Inhibitor AX-0085 Inhibits Interferon-gamma-Mediated Induction of PD-L1 Expression and Promotes Immune Reaction to Lung Adenocarcinoma Cells
    Authors: J Kim, H Jang, GJ Lee, Y Hur, J Keum, JK Jo, SE Yun, SJ Park, YJ Park, MJ Choi, KS Kim, J Kim
    Cells, 2021-12-22;11(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Loss of H2R signaling disrupts neutrophil homeostasis and promotes inflammation-associated colonic tumorigenesis in mice
    Authors: Z Shi, Y Mori-Akiya, W Du, R Fultz, Y Zhao, W Ruan, S Venable, MA Engevik, J Versalovic
    Cellular and Molecular Gastroenterology and Hepatology, 2021-11-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Immune receptor inhibition through enforced phosphatase recruitment
    Authors: RA Fernandes, L Su, Y Nishiga, J Ren, AM Bhuiyan, N Cheng, CJ Kuo, LK Picton, S Ohtsuki, RG Majzner, SP Rietberg, CL Mackall, Q Yin, LR Ali, X Yang, CS Savvides, J Sage, M Dougan, KC Garcia
    Nature, 2020-10-21;586(7831):779-784.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CD47 prevents the elimination of diseased fibroblasts in scleroderma
    Authors: T Lerbs, L Cui, ME King, T Chai, C Muscat, L Chung, R Brown, K Rieger, T Shibata, G Wernig
    JCI Insight, 2020-08-20;5(16):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  15. Impacts of combining anti-PD-L1 immunotherapy and radiotherapy on the tumour immune microenvironment in a murine prostate cancer model
    Authors: Y Philippou, HT Sjoberg, E Murphy, S Alyacoubi, KI Jones, AN Gordon-Wee, S Phyu, EE Parkes, W Gillies Mc, AD Lamb, U Gileadi, V Cerundolo, DA Scheiblin, SJ Lockett, DA Wink, IG Mills, FC Hamdy, RJ Muschel, RJ Bryant
    Br. J. Cancer, 2020-07-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer
    Authors: LM Schreiber, C Urbiola, K Das, B Spiesschae, J Kimpel, F Heinemann, B Stierstorf, P Müller, M Petersson, P Erlmann, D von Laer, G Wollmann
    Br. J. Cancer, 2019-09-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer
    Authors: F Ritzmann, C Jungnickel, G Vella, A Kamyschnik, C Herr, D Li, MM Menger, A Angenendt, M Hoth, A Lis, R Bals, C Beisswenge
    Sci Rep, 2019-07-17;9(1):10353.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Interferon-gamma drives programmed death-ligand 1 expression on islet ? cells to limit T cell function during autoimmune diabetes
    Authors: KC Osum, AL Burrack, T Martinov, NL Sahli, JS Mitchell, CG Tucker, KE Pauken, K Papas, B Appakalai, JA Spanier, BT Fife
    Sci Rep, 2018-05-29;8(1):8295.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. PD-L1 checkpoint inhibition and anti-CTLA-4 whole tumor cell vaccination counter adaptive immune resistance: A mouse neuroblastoma model that mimics human disease
    Authors: P Srinivasan, X Wu, M Basu, C Rossi, AD Sandler
    PLoS Med., 2018-01-29;15(1):e1002497.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Hormonal vitamin D upregulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in human but not mouse
    Authors: V Dimitrov, M Bouttier, G Boukhaled, R Salehi-Tab, R Avramescu, B Memari, B Hasaj, GL Lukacs, CM Krawczyk, JH White
    J. Biol. Chem., 2017-10-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Tumor localized secretion of soluble PD1 enhances oncolytic virotherapy
    Authors: MY Bartee, KM Dunlap, E Bartee
    Cancer Res, 2017-03-17;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  23. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas
    Int J Cancer, 2016-08-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors
    Clin Cancer Res, 2016-07-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. IFN-gamma from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer.
    Authors: Abiko K, Matsumura N, Hamanishi J, Horikawa N, Murakami R, Yamaguchi K, Yoshioka Y, Baba T, Konishi I, Mandai M
    Br J Cancer, 2015-03-31;112(9):1501-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  26. beta-Cell-targeted blockage of PD1 and CTLA4 pathways prevents development of autoimmune diabetes and acute allogeneic islets rejection.
    Authors: El Khatib M, Sakuma T, Tonne J, Mohamed M, Holditch S, Lu B, Kudva Y, Ikeda Y
    Gene Ther, 2015-03-19;22(5):430-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2012-09-27;133(2):334-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010-02-05;184(5):2337-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  29. Altered availability of PD-1/PD ligands is associated with the failure to control autoimmunity in NOD mice.
    Authors: Yadav D, Hill N, Yagita H, Azuma M, Sarvetnick N
    Cell. Immunol., 2009-05-06;258(2):161-71.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. Establishment of NOD-Pdcd1-/- mice as an efficient animal model of type I diabetes.
    Authors: Wang J, Yoshida T, Nakaki F, Hiai H, Okazaki T, Honjo T
    Proc. Natl. Acad. Sci. U.S.A., 2005-08-08;102(33):11823-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Secondary Antibodies

Goat IgG (H+L) Antibody

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Reviews for Mouse PD-L1/B7-H1 Antibody

Average Rating: 4.4 (Based on 10 Reviews)

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Mouse PD-L1 Antibody
By Anonymous on 07/31/2020
Application: IHC Sample Tested: Breast cancer tissue Species: Mouse

Mouse PD-L1 Antibody
By Shubhankar Ambike on 01/21/2020
Application: Flow Sample Tested: MH-S mouse macrophage cell line,RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse

MH-S and RAW264.7 cells were treated with 10ug/ml concentration of IFN alpha. Cells were analyzed by flow cytometer (surface staining) for PD-L1 expression using a labelled secondary antibody. Protocol was followed as given with the article. Worked exactly as expected.


Mouse PD-L1 Antibody
By Shubhankar Ambike on 12/31/2019
Application: WB Sample Tested: Cell Lysates Species: Mouse

MH-S mice macrophage cell lysates. Dark bands in the middle are PD-L1


Mouse PD-L1 Antibody
By Anonymous on 03/20/2019
Application: WB Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

Mouse PD-L1 Antibody
By Dong Hu on 02/28/2019
Application: WB Sample Tested: mouse beast cancer cell line Eo771 Species: Mouse

Mouse PD-L1 Antibody
By Anonymous on 10/28/2018
Application: WB Sample Tested: 4T1 mouse breast cancer cell line,EMT6 cells Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Anonymous on 03/22/2018
Application: IHC Sample Tested: frozen section Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Angelo Taglialatela on 11/26/2017
Application: WB Sample Tested: cultured mouse mammary gland tumor,mouse mammary gland tumor cells Species: Mouse

Technical Service will be following up with the customer.


Mouse PD-L1/B7-H1 Antibody
By Purushottam Lamichhane on 10/17/2017
Application: IHC Sample Tested: Pancreas tissue Species: Mouse

Mouse B7‑H1/PD‑L1 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse lymphocytes Species: Mouse

Buffer: tbst
Dilution: 1/1000