Mouse/Rat P-Selectin/CD62P Antibody

Catalog # Availability Size / Price Qty
AF737
AF737-SP
Detection of Mouse and Rat P-Selectin/CD62P by Western Blot.
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Product Details
Citations (12)
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Reviews (1)

Mouse/Rat P-Selectin/CD62P Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62P
Trp42-Ala709
Accession # Q01102
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat P-Selectin/CD62P antibody by Western Blot. View Larger

Detection of Mouse and Rat P-Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry P-Selectin/CD62P antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

P‑Selectin/CD62P in Mouse Liver. P-Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Rat P-Selectin/CD62P antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat P-Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P-Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence In-vivo targeting of BYL719-loaded nanoparticles prepared with either fucoidan (Fi) or dextran sulfate (Dex).(a) Representative ex vivo fluorescence images of mice organs 24 h after i.v. administration of FiBYL719 or DexBYL719 nanoparticles, and pre-treated with anti-P-selectin antibody (Ab). (b) Nanoparticle biodistribution in organs and tumour, calculated from ex vivo fluorescence images shown in a as total fluorescence efficiency divided by organ weight (n=3). (c) Representative immunofluorescence staining for CD-31 (red), P-selectin (green) and DAPI (blue) in Cal-33 xenografts before and after a single dose of ionizing radiation (4 Gy). Scale bars, 50 μm. (d) Quantification of double-staining positive endothelial cells per tumour shown in c (n=3). (e) In vivo fluorescence imaging of Cal-33 xenograft-bearing mice 24 h after treatment with FiBYL719 or 4 Gy RT followed by FiBYL719. (f) Quantification of total fluorescence efficiency of tumours shown e (n=10). (g) Representative immunofluorescence stains of tumour sections for P-selectin (green), NIR (red) and DAPI (blue) from H22 xenografts 24 h after treatments. Scale bars, 50 μm. In b,d,f error bars indicate mean±s.e.m. *P<0.05, **P<0.01; by Mann–Whitney U-test in b or one-way ANOVA with post hoc Tukey test in d,f. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms14292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence In-vivo targeting of BYL719-loaded nanoparticles prepared with either fucoidan (Fi) or dextran sulfate (Dex).(a) Representative ex vivo fluorescence images of mice organs 24 h after i.v. administration of FiBYL719 or DexBYL719 nanoparticles, and pre-treated with anti-P-selectin antibody (Ab). (b) Nanoparticle biodistribution in organs and tumour, calculated from ex vivo fluorescence images shown in a as total fluorescence efficiency divided by organ weight (n=3). (c) Representative immunofluorescence staining for CD-31 (red), P-selectin (green) and DAPI (blue) in Cal-33 xenografts before and after a single dose of ionizing radiation (4 Gy). Scale bars, 50 μm. (d) Quantification of double-staining positive endothelial cells per tumour shown in c (n=3). (e) In vivo fluorescence imaging of Cal-33 xenograft-bearing mice 24 h after treatment with FiBYL719 or 4 Gy RT followed by FiBYL719. (f) Quantification of total fluorescence efficiency of tumours shown e (n=10). (g) Representative immunofluorescence stains of tumour sections for P-selectin (green), NIR (red) and DAPI (blue) from H22 xenografts 24 h after treatments. Scale bars, 50 μm. In b,d,f error bars indicate mean±s.e.m. *P<0.05, **P<0.01; by Mann–Whitney U-test in b or one-way ANOVA with post hoc Tukey test in d,f. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms14292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

References
  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citations for Mouse/Rat P-Selectin/CD62P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. A dhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T�cells with enhanced tumor homing to improve cell therapy
    Authors: CP Camargo, AK Muhuri, Y Alapan, LF Sestito, M Khosla, MP Manspeaker, AS Smith, CM Paulos, SN Thomas
    Cell Reports, 2023-02-26;42(3):112175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activation
    Authors: P Marona, J Górka, O Kwapisz, J Jura, J Rys, RM Hoffman, K Miekus
    Cell Death & Disease, 2022-09-22;13(9):814.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasis
    Authors: H Li, Z Wang, Z Chen, T Ci, G Chen, D Wen, R Li, J Wang, H Meng, R Bryan Bell, Z Gu, G Dotti, Z Gu
    Nature Communications, 2021-05-13;12(1):2773.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS
    Authors: Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo
    J. Clin. Invest., 2020-04-01;0(0):.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC-P
  5. Alcohol exposure-induced neurovascular inflammatory priming impacts ischemic stroke and is linked with brain perivascular macrophages
    Authors: A Drieu, A Lanquetin, D Levard, M Glavan, F Campos, A Quenault, E Lemarchand, M Naveau, AL Pitel, J Castillo, D Vivien, M Rubio
    JCI Insight, 2020-02-27;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, In Vivo
  6. Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation
    Authors: G Xu, C Li, AL Parsiola, J Li, KD McCarter, R Shi, WG Mayhan, H Sun
    Front Cell Neurosci, 2019-02-12;13(0):6.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin
    Authors: AP Fournier, A Quenault, S Martinez d, M Gauberti, G Defer, D Vivien, F Docagne, R Macrez
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma
    Authors: A Mizrachi, Y Shamay, J Shah, S Brook, J Soong, VK Rajasekhar, JL Humm, JH Healey, SN Powell, J Baselga, DA Heller, A Haimovitz-, M Scaltriti
    Nat Commun, 2017-02-13;8(0):14292.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack
    Authors: Aurélien Quenault
    Brain, 2016-11-08;140(0):146-157.
    Species: Mouse
    Sample Types: Complex Sample Type, Whole Tissue
    Applications: Functional Assay, IHC
  10. Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
    Authors: Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ
    Atherosclerosis, 2008-02-21;199(1):55-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
    Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
    Atherosclerosis, 2008-02-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets.
    Authors: Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, McVicar DW
    Blood, 2004-04-20;104(4):1042-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse/Rat P-Selectin/CD62P Antibody

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Mouse/Rat P-Selectin/CD62P Antibody
By Anonymous on 01/22/2018
Application: IHC Sample Tested: Brain tissue,Spinal cord Species: Mouse

dilution: 1/1000