Mouse/Rat Tie-2 Antibody Summary
Ala23-Lys744
Accession # CAA47857
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Tie-2 in Mouse Intestine Tissue. Tie-2 was detected in immersion fixed paraffin-embedded sections of mouse intestine tissue using Goat Anti-Mouse/Rat Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF762) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
Product Datasheets
Citations for Mouse/Rat Tie-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc
Authors: YW Koo, CS Lim, A Darai, J Lee, W Kim, I Han, GH Kim
Biomaterials research, 2023-03-29;27(1):26.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration
Authors: K Shirakura, P Baluk, AF Nottebaum, U Ipe, KG Peters, DM McDonald, D Vestweber
Embo Molecular Medicine, 2023-02-06;0(0):e16128.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice
Authors: A Lask, B Gutbier, O Kershaw, G Nouailles, AD Gruber, HC Müller-Red, S Chackowicz, DA Hamilton, P Van Slyke, M Witzenrath
Scientific Reports, 2022-09-15;12(1):15531.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Monocyte pro-inflammatory phenotypic control by Ephrin type-A receptor 4 mediates neural tissue damage
Authors: EA Kowalski, E Soliman, C Kelly, EK Gudenschwa, J Leonard, KJ Pridham, J Ju, AM Cash, A Hazy, C de Jager, AM Kaloss, H Ding, RD Hernandez, GM Coleman, X Wang, ML Olsen, AM Pickrell, MH Theus
JCI Insight, 2022-08-08;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment
Authors: S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam
Nature Communications, 2022-06-20;13(1):3535.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
Species: Mouse
Sample Types: Protein Lysates, Whole Tissue
Applications: IHC, Western Blot -
An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1
Authors: B Lee, H Shin, JE Oh, J Park, M Park, SC Yang, JH Jun, SH Hong, H Song, HJ Lim
Autophagy, 2020-06-17;0(0):1-18.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency
Authors: A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng
Int J Mol Sci, 2020-05-25;21(10):.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Perivascular Stem Cell-Derived Cyclophilin A Improves Uterine Environment with Asherman's Syndrome via HIF1alpha-Dependent Angiogenesis
Authors: M Park, SH Hong, SH Park, YS Kim, SC Yang, HR Kim, S Noh, S Na, HK Lee, HJ Lim, SW Lyu, H Song
Mol. Ther., 2020-05-20;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy
Authors: W Dong, R Li, H Yang, Y Lu, L Zhou, L Sun, D Wang, J Duan
Sci Rep, 2020-04-20;10(1):6652.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model
Authors: H Chen, X Fu, J Jiang, S Han
Front Pharmacol, 2019-12-03;10(0):1373.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861
Authors: AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D
Cancer Res., 2019-06-12;0(0):.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
Sci Adv, 2019-02-13;5(2):eaau6732.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Pulmonary pericytes regulate lung morphogenesis
Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
Nat Commun, 2018-06-22;9(1):2448.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Tie2 protects the vasculature against thrombus formation in systemic inflammation
Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
J. Clin. Invest., 2018-03-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
J. Clin. Invest., 2017-09-18;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
Circ. Res, 2017-02-08;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
Authors: Zhou Y, Williams J, Smallwood P, Nathans J
PLoS ONE, 2015-12-02;10(12):e0143650.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nat Commun, 2015-01-30;6(0):5962.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
COMP-angiopoietin-1 recovers molecular biomarkers of neuropathy and improves vascularisation in sciatic nerve of ob/ob mice.
Authors: Kosacka J, Nowicki M, Kloting N, Kern M, Stumvoll M, Bechmann I, Serke H, Bluher M
PLoS ONE, 2012-03-06;7(3):e32881.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
Authors: Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
Biochem. Biophys. Res. Commun., 2008-11-24;378(2):296-301.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A neurovascular niche for neurogenesis after stroke.
Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
J. Neurosci., 2006-12-13;26(50):13007-16.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Immunotherapy of tumors with protein vaccine based on chicken homologous Tie-2.
Authors: Luo Y, Wen YJ, Ding ZY, Fu CH, Wu Y, Liu JY, Li Q, He QM, Zhao X, Jiang Y, Li J, Deng HX, Kang B, Mao YQ, Wei YQ
Clin. Cancer Res., 2006-03-15;12(6):1813-9.
Species: Chicken, Mouse
Sample Types: Recombinant Protein
Applications: Western Blot
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Unfixed cryosections were fixed in 95% EtOH followed by acetone before blocking and overnight incubation in 1:50 AF762 at 4C. Staining was detected with Alexafluor-488 labeled donkey anti-goat secondary AB. Strong staining was observed in mouse kidney sections.
