Mouse SCF/c-kit Ligand Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SCF/c-kit Ligand in ELISAs and Western blots. In Western blots, less than 20% cross-reactivity with recombinant human SCF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse SCF/c-kit Ligand
    Lys26-Ala189
    Accession # Q78ED8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC)
    • Mouse SCF/c-kit Ligand Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse SCF/c‑kit Ligand Antibody (Catalog # AF-455-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF455)
  • ELISA Standard
     
    Recombinant Mouse SCF Protein (Catalog # 455-MC)
  • Neutralization
    Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.3-0.6 µg/mL in the presence of 25 ng/mL Recombinant Mouse SCF/c‑kit Ligand.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Mouse SCF/c‑kit Ligand Antibody. Recombinant Mouse
SCF/c‑kit Ligand (Catalog #
455‑MC) stimulates prolif­eration in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse
SCF/c‑kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of
Goat Anti-Mouse
SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND50 is typically 0.3‑0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

  • References:
    1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
    2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
    3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
    4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
    5. Kapur, R. et al. (2002) Blood 100:1287.
    6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
    7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
    8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
    9. Huang, E. et al. (1990) Cell 63:225.
    10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
    11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
    12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4671.
    13. Flanagan, J.G. et al. (1991) Cell 64:1025.
    14. Martin, F.H. et al. (1990) Cell 63:203.
    15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
    16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  • Long Name:
    Stem Cell Factor
  • Entrez Gene IDs:
    4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
  • Alternate Names:
    c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  2. Steel factor controls primordial germ cell survival and motility from the time of their specification in the allantois, and provides a continuous niche throughout their migration.
    Authors: Gu Y, Runyan C, Shoemaker A, Surani A, Wylie C
    Development, 2009;136(8):1295-303.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling.
    Authors: Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH
    Am. J. Pathol., 2009;174(2):390-400.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  4. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
    Authors: Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
    Blood, 2008;112(4):1269-79.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Nociceptive tuning by stem cell factor/c-Kit signaling.
    Authors: Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN
    Neuron, 2007;56(5):893-906.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. Indispensable role of Bcl2 in the development of the melanocyte stem cell.
    Authors: Mak SS, Moriyama M, Nishioka E, Osawa M, Nishikawa S
    Dev. Biol., 2006;291(1):144-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Chicken Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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