Mouse SCF/c-kit Ligand Antibody

Catalog # Availability Size / Price Qty
AF-455-NA
AF-455-SP
Mouse SCF/c-kit Ligand Antibody in Data
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Citations (8)
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Mouse SCF/c-kit Ligand Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SCF/c-kit Ligand in ELISAs and Western blots. In Western blots, less than 20% cross-reactivity with recombinant human SCF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse SCF/c-kit Ligand
Lys26-Ala189
Accession # Q78ED8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC)

Mouse SCF/c-kit Ligand Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse SCF/c-kit Ligand Biotinylated Antibody (Catalog # BAF455)

Standard: Recombinant Mouse SCF Protein (Catalog # 455-MC)

Neutralization
Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.3-0.6 µg/mL in the presence of 25 ng/mL Recombinant Mouse SCF/c‑kit Ligand.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Mouse SCF/c‑kit Ligand Antibody. View Larger

Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Mouse SCF/c‑kit Ligand Antibody. Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND50is typically 0.3-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

References
  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287.
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
  9. Huang, E. et al. (1990) Cell 63:225.
  10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4671.
  13. Flanagan, J.G. et al. (1991) Cell 64:1025.
  14. Martin, F.H. et al. (1990) Cell 63:203.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Long Name
Stem Cell Factor
Entrez Gene IDs
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Product Datasheets

Citations for Mouse SCF/c-kit Ligand Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  2. Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling.
    Authors: Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH
    Am. J. Pathol., 2009;174(2):390-400.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  3. Steel factor controls primordial germ cell survival and motility from the time of their specification in the allantois, and provides a continuous niche throughout their migration.
    Authors: Gu Y, Runyan C, Shoemaker A, Surani A, Wylie C
    Development, 2009;136(8):1295-303.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
    Authors: Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
    Blood, 2008;112(4):1269-79.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Nociceptive tuning by stem cell factor/c-Kit signaling.
    Authors: Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN
    Neuron, 2007;56(5):893-906.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Indispensable role of Bcl2 in the development of the melanocyte stem cell.
    Authors: Mak SS, Moriyama M, Nishioka E, Osawa M, Nishikawa S
    Dev. Biol., 2006;291(1):144-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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