Mouse VCAM-1/CD106 Antibody
Mouse VCAM-1/CD106 Antibody Summary
Phe25-Glu698
Accession # P29533
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-specific_Demethylase_4A_AF6434_Western_Blot_10062.jpg "Detection of Human and Mouse Lysine (K)-specific Demethylase 4A/KDM4A antibody by Western Blot.")
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VCAM-1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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VCAM‑1/CD106 in Mouse Embryo. VCAM-1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of VCAM‑1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse VCAM-1/CD106 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
21
Citations: Showing 1 - 10
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The protective role of proton-sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model
Authors: K Sato, A Tobo, C Mogi, M Tobo, N Yamane, M Tosaka, H Tomura, DS Im, F Okajima
Sci Rep, 2020;10(1):17193.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
Exp. Mol. Med., 2020;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Authors: S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin
Dis Model Mech, 2020;13(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIb?
Authors: JA Giles, AD Greenhalgh, A Denes, B Nieswandt, G Coutts, BW McColl, SM Allan
Immunology, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
Authors: P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein
Front Physiol, 2018;9(0):1754.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
Elife, 2018;7(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
J Am Heart Assoc, 2017;6(12):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Loss of Ptpn11 (Shp2) drives satellite cells into quiescence
Authors: J Griger, R Schneider, I Lahmann, V Schöwel, C Keller, S Spuler, M Nazaré, C Birchmeier
Elife, 2017;6(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Numb regulates somatic cell lineage commitment during early gonadogenesis in mice
Authors: YT Lin, L Barske, T DeFalco, B Capel
Development, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction
Authors: N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury
JCI Insight, 2017;2(17):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Cell Selection -
Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
J Immunol, 2015;194(11):5061-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
Authors: Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa J, Chlopicki S, Nachtigal P
PLoS ONE, 2015;10(3):e0119665.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
Authors: Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A
Circulation, 2015;131(6):560-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
Authors: Ferrer M, Shuvaev V, Zern B, Composto R, Muzykantov V, Eckmann D
PLoS ONE, 2014;9(7):e102329.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
PLoS ONE, 2013;8(12):e84479.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Targeting improves MSC treatment of inflammatory bowel disease.
Authors: Ko IK, Kim BG, Awadallah A
Mol. Ther., 2010;18(7):1365-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
EMBO J., 2009;28(9):1319-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
High pressure promotes monocyte adhesion to the vascular wall.
Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circ. Res., 2007;100(8):1226-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
Circulation, 2007;117(3):421-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
Authors: Nguyen M, Pace AJ, Koller BH
J. Exp. Med., 2007;204(6):1383-93.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood, 2007;109(9):3706-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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