Mouse VCAM-1/CD106 Antibody

(12 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VCAM‑1/CD106 in direct ELISAs and Western blots.  In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VCAM‑1/CD106
    Phe25-Glu698
    Accession # P29533
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Mouse VCAM-1/CD106 Fc Chimera (Catalog # 643-VM, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 50 µg/mL of the antibody.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VCAM‑1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of VCAM‑1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunohistochemistry
VCAM‑1/CD106 in Mouse Embryo. VCAM‑1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

  • Long Name:
    Vascular Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    7412 (Human); 22329 (Mouse)
  • Alternate Names:
    CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Loss of Ptpn11 (Shp2) drives satellite cells into quiescence
    Authors: J Griger, R Schneider, I Lahmann, V Schöwel, C Keller, S Spuler, M Nazaré, C Birchmeier
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
    Authors: Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A
    Circulation, 2015;131(6):560-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  3. High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
    Authors: Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa J, Chlopicki S, Nachtigal P
    PLoS ONE, 2015;10(3):e0119665.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
    Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
    J Immunol, 2015;194(11):5061-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
    Authors: Ferrer M, Shuvaev V, Zern B, Composto R, Muzykantov V, Eckmann D
    PLoS ONE, 2014;9(7):e102329.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
    Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
    PLoS ONE, 2013;8(12):e84479.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Targeting improves MSC treatment of inflammatory bowel disease.
    Authors: Ko IK, Kim BG, Awadallah A
    Mol. Ther., 2010;18(7):1365-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
    Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
    EMBO J., 2009;28(9):1319-31.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007;100(8):1226-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
    Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
    Circulation, 2007;117(3):421-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
    Authors: Nguyen M, Pace AJ, Koller BH
    J. Exp. Med., 2007;204(6):1383-93.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 12 Citations
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