Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 55010
View all Wnt-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724

Specificity

Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse Wnt‑4 Antibody

Wnt-4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Wnt‑4 in Mouse Embryo.

Wnt-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Human Wnt-4 ELISA Standard Curve.

Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human/Mouse Wnt‑4 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody for Human Wnt-4 when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Wnt‑4 (Catalog # 475-WN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-4

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Alternate Names

Wnt4

Entrez Gene IDs

54361 (Human); 22417 (Mouse)

Gene Symbol

WNT4

UniProt

P22724

Additional Wnt-4 Products

Product Documents for Human/Mouse Wnt‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Wnt‑4 Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Citations for Human/Mouse Wnt‑4 Antibody

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Protocols

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FAQs

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