Wnt‑4 in Mouse Embryo.
Wnt‑4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse Wnt‑4 Monoclonal Antibody (Catalog # MAB475) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Wnt-4 is a secreted glycoprotein that binds to receptors of the
Frizzled family. Wnt-4 is important for multiple embryonic development
processes including kidney morphogenesis.
Wingless-type MMTV Integration Site Family, Member 4
Entrez Gene IDs:
54361 (Human); 22417 (Mouse)
protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4
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