N-Acetylcysteine

Tocris Bioscience | Catalog # 7874

Glutathione (GSH) precursor; maintains stem cell function in culture.
Tocris Bioscience

Key Product Details

Description

Glutathione (GSH) precursor; maintains stem cell function in culture.
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Product Description

N

-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.

Scientific Data Images for N-Acetylcysteine

Adult Stem Cell-derived Lung Organoids

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Adult Stem Cell-derived Liver Organoids

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Liver Organoids.

Adult stem cell-derived liver organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), Forskolin (Catalog # 1099), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes N-Acetylcysteine, HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

iPSC-derived Human Intestinal Organoid

Application of N-Acetylcysteine in Culture and Characterization of iPSC-derived Human Intestinal Organoids.

iPSC-derived human intestinal organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).

Adult Stem Cell-derived Human Descending Colon Organoids

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids.

Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with Anti-MUC2 (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). (B) Organoids were fixed and stained with Human Chromogranin A Antibody(Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue).

Product Specifications for N-Acetylcysteine

Molecular Weight

163.20

Formula

C5H9NO3S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(2R)-2-Acetamido-3-sulfanylpropanoic acid

CAS Number

616-91-1

PubChem ID

12035

InChI Key

PWKSKIMOESPYIA-BYPYZUCNSA-N

SMILES

[C@@H](NC(C)=O)(C(O)=O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 8.16 50
DMSO 8.16 50

Preparing Stock Solutions for N-Acetylcysteine

The following data is based on the product molecular weight 163.20.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 12.25 mL 61.27 mL 122.55 mL
2.5 mM 2.45 mL 12.25 mL 24.51 mL
5 mM 1.23 mL 6.13 mL 12.25 mL
25 mM 0.25 mL 1.23 mL 2.45 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 60 publications citing the usage of this product.

Product Documents for N-Acetylcysteine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for N-Acetylcysteine

For research use only

Citations for N-Acetylcysteine

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  • test
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Gastric cancer cells (AGS)
    Verified Customer | Posted 08/18/2025
    NAC is water soluble. Made a 1000x stock solution and add it directly to the culture medium.
    NAC was used to inhibit ROS overproduction by Helicobacter pylori (H. pylori) injection in gastric cancer cells AGS. The use of ROS quencher NAC was to investigate the involvement of H.pylori-induced ROS overproduction in epithelial-mesenchymal transition of gastric cancer cells AGS.
    N-Acetylcysteine 7874

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